Simultaneous determination of six bioactive flavonoids in Citri Reticulatae Pericarpium by rapid resolution liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry
Simultaneous determination of six bioactive flavonoids in Citri Reticulatae Pericarpium by rapid resolution liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry
复制标题
快速分辨液相色谱-三重四极杆电喷雾串联质谱法同时测定陈皮中六种生物活性黄酮类成分
DOI：
10.1016/j.foodchem.2013.06.077
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发表时间：
2013-12-15
期刊：
影响因子：
8.8
通讯作者：
Li, Ping
中科院分区：
文献类型：
--
作者：
Liu, E-Hu;Zhao, Pan;Li, Ping
建立了快速分离液相色谱/电喷雾串联质谱（RRLC-ESI-MSn）法，并对该方法进行了验证。采用Cell Counting Kit-8法对C-反应蛋白中的6种黄酮类化合物（柚皮苷、橙皮苷、川陈皮、3，5，6，7，8，3 '，4'-七甲氧基黄酮、橘子苷和5-羟基-6，7，8，3 '，4'-五甲氧基黄酮）的抗增殖活性进行评价和比较。使用多反应监测模式在Agilent三重四极杆LC-MS系统上进行定量。该方法已成功地应用于我国不同地区样品中6种黄酮类化合物的测定。与已报道的分析方法相比，RALC-ESI-MSn方法在多组分定量分析方面具有快速、灵敏等优点。并根据6种特征性黄酮类化合物的含量进行系统聚类分析。HCA结果表明，柑橘'茶池'样品可以很容易地从其他CRP样品区分。建立的RRLC-ESI-MSn方法结合HCA可作为CRP的质量控制方法。(C)2013爱思唯尔有限公司保留所有权利。
A rapid resolution liquid chromatography/electrospray ionisation tandem mass spectrometry (RRLC-ESI-MSn) method has been firstly developed and validated for simultaneous determination of six bioactive flavonoids in Citri Reticulatae Pericarpium (CRP). The antiproliferative activities of the six flavonoids in CRP, namely naringin, hesperidin, nobiletin, 3,5,6,7,8,3',4'-heptamethoxyflavone, tangeretin and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone, were evaluated and compared by Cell Counting Kit-8 Assay. Quantification was carried out on an Agilent triple quadrupole LC-MS system using multiple reaction monitoring mode. The established method was successfully applied for determination of the six flavonoids in samples collected from different regions in China. Compared with the reported analytical methods, the RALC-ESI-MSn method is powerful in quantitative analysis of multi-component in terms of time savings and sensitivity. Hierarchical cluster analysis (HCA) was also performed to differentiate and classify the samples based on the contents of the six characteristic flavonoids. The HCA results indicated that Citrus reticulata 'Chachi' samples could be easily distinguished from other CRP samples. The developed RRLC-ESI-MSn method combined with HCA might be utilised as a quality control method for CRP. (C) 2013 Elsevier Ltd. All rights reserved.