HORTICULTURAL CHARACTERISTICS OF TRANSGENIC TOBACCO EXPRESSING THE ROLC GENE FROM AGROBACTERIUM RHIZOGENES
HORTICULTURAL CHARACTERISTICS OF TRANSGENIC TOBACCO EXPRESSING THE ROLC GENE FROM AGROBACTERIUM RHIZOGENES
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表达发根农杆菌 ROLC 基因的转基因烟草的园艺特性
DOI：
10.21273/jashs.119.5.1091
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发表时间：
1994
影响因子：
1.9
通讯作者：
M. Ravelonandro
中科院分区：
文献类型：
--
作者：
R. Scorza;T. Zimmerman;J. Cordts;K. J. Footen;M. Ravelonandro
威斯康星州38号烟草(Nictiana tabacum L.)将已解除武装的根癌农杆菌菌株EHA101(Oono et al.，1987)转化叶圆盘，获得了携带发根农杆菌RolC基因、NPT II和GUS基因的转基因植株。通过GUS检测、聚合酶链式反应(PCR)、Southern杂交和外源基因在有性周期中的传递，证实了在含卡那霉素的培养基上再生的不定芽是转基因的。转基因植株的高度只有对照植株的一半，开花时间提前了35天，而且叶片更小，节间更短，种皮更小，种子更少，花更小，花粉活力降低。转基因植株的种皮数、叶片数和比根长与对照植株相似。转基因无性系在RolC诱导的生长变化的表达上有所不同，这些无性系的第一代幼苗也是如此。这些差异暗示了为不同表达水平进行选择的可能性。用RolC基因转化园艺作物是一种潜在的有用的遗传改良方法，特别是在花期、高度、叶片和花的大小方面。化学名称：新霉素磷酸转移酶(NPTⅡ)、β-葡萄糖醛酸苷酶(GUS)。改变植株形态是园艺作物育种计划的一个主要目标。缩小植株大小对从果树到一年生铺垫植物的各种作物都很有用。控制花朵大小是观赏作物育种计划的重要组成部分。大多数育种计划依赖于杂交和选择来改变植物和花朵的形状，尽管在某些情况下使用了辐射(Micke等人，1987)。
Wisconsin 38' tobacco ( Nicotiana tabacum L.) leaf discs were transformed with the disarmed Agrobacterium tumefaciens strain EHA101 carrying the rolC gene from A. rhizogenes (Oono et al., 1987) and NPT II and GUS genes. Shoots that regenerated on kanamycin-containing medium were confirmed as transgenic through GUS assays, polymerase chain reaction (PCR), Southern blot analyses, and transmission of the foreign genes through the sexual cycle. Transgenic plants were as short as half the height of control plants; were earlier flowering by up to 35 days; and had smaller leaves, shorter internodes, smaller seed capsules, fewer seeds, smaller flowers, and reduced pollen viability. The number of seed capsules, leaf number, and specific root length were similar between transgenic and control plants. Transgenic clones varied in the expression of the rolC-induced growth alterations as did the first generation of seedlings from these clones. Such differences suggested the potential for selecting for different levels of expression. Transformation with the rolC gene presents a potentially useful method of genetically modifying horticultural crops, particularly for flowering date, height, and leaf and flower size. Chemical names used: neomycin phosphotransferase (NPTII), β-glucuronidase (GUS). Altering plant form is a major goal of breeding programs for horticultural crops. Reduced plant size is useful in crops ranging from tree fruit to annual bedding plants. Manipulating flower size is an important component of ornamental crop breeding programs. Most breeding programs depend on hybridization and selection to alter plant and flower form, although irradiation has been used in some cases (Micke et al., 1987).