A Novel Method for In Vivo Imaging of Solitary Lung Nodules Using Navigational Bronchoscopy and Confocal Laser Microendoscopy
A Novel Method for In Vivo Imaging of Solitary Lung Nodules Using Navigational Bronchoscopy and Confocal Laser Microendoscopy
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DOI：
10.1007/s00408-015-9769-8
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发表时间：
2015-10-01
期刊：
影响因子：
5
通讯作者：
Thiberville, L.
中科院分区：
文献类型：
--
作者：
Hassan, T.;Piton, N.;Thiberville, L.
孤立性肺结节（SPN）越来越普遍，诊断管理仍然具有挑战性。我们展示了一种新的技术，其中探针为基础的共聚焦显微内镜（pCLE）可以进行显微成像SPN在体内和实时。两个共焦波长（488和660 nm与亚甲蓝（MB））用于弹性蛋白网络和细胞成像，分别使用pCLE结合r-EBUS和虚拟导航。在第一个病例中，使用1 mm Alveoflex（A（R））对右中叶亚厘米级结节中的转移性黑色素瘤进行成像。在下一个病例中，使用较小的0.6 mm Cholangioflex（A（R））对上叶后段的一个2 cm恶性结节进行成像。最后，我们提出了一个良性的情况下，揭示共焦特征的结节性脂质性肺炎。这是第一次报告的可行性和实用性的pCLE在SPN的体内显微成像使用Alveoflex或Cholangioflex微型探针除了660 nm/MB成像。
Solitary pulmonary nodules (SPN) have become increasingly prevalent and diagnostic management remains challenging. We demonstrate a novel technique in which probe-based confocal endomicroscopy (pCLE) could be performed to microimage SPN in vivo and in real-time. Two confocal wavelengths (488 and 660 nm with methylene blue (MB)) were used for elastin network and cellular imaging, respectively using pCLE in conjunction with r-EBUS and virtual navigation. In the first case, the 1-mm Alveoflex(A (R)) was used to image a metastatic melanoma in a subcentimetric nodule in the right middle lobe. In the next case, a malignant 2-cm nodule in the posterior segment of the upper lobe was imaged using the smaller 0.6-mm Cholangioflex(A (R)). Lastly, we present a benign case revealing confocal characteristics of a nodular lipid pneumonitis. This reports for the first time the feasibility and utility of pCLE in vivo microimaging of SPN using either the Alveoflex or Cholangioflex miniprobes in addition to 660 nm/MB imaging.