BCLX REGULATES THE SURVIVAL OF DOUBLE-POSITIVE THYMOCYTES
BCLX REGULATES THE SURVIVAL OF DOUBLE-POSITIVE THYMOCYTES
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DOI：
10.1073/pnas.92.11.4763
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发表时间：
1995-05-23
影响因子：
11.1
通讯作者：
THOMPSON, CB
中科院分区：
文献类型：
--
作者：
MA, A;PENA, JC;THOMPSON, CB
bclx基因已被证明在体外调节程序性细胞死亡。我们现在发现，当T细胞从CD 4（-）CD 8（-）（双阴性）胸腺细胞分化为CD 4（+）CD 8（+）[双阳性（DP）]胸腺细胞时，Bclx表达显著增加。相反，单阳性（SP）胸腺细胞表达可忽略不计的Bclx蛋白。这种表达模式与Bcl 2的表达模式形成对比，Bcl 2存在于双阴性胸腺细胞中，在DP胸腺细胞中下调，并在成熟后重新诱导为SP胸腺细胞。通过基因靶向消除Bclx显著缩短DP胸腺细胞的存活，但不缩短SP胸腺细胞或外周SP T细胞的存活。这些数据表明，在胸腺成熟过程中Bclx的诱导在调节DP胸腺细胞在没有选择的情况下存活的时间长度中起关键作用。
The bclx gene has been shown to regulate programmed cell death in vitro. We now show that Bclx expression increases dramatically when T cells differentiate from CD4(-) CD8(-) (double negative) thymocytes to CD4(+) CD8(+) [double positive (DP)] thymocytes, In contrast single-positive (SP) thymocytes express negligible amounts of Bclx protein. This expression pattern contrasts with that of Bcl2, which is present in double-negative thymocytes, downregulated in DP thymocytes, and reinduced upon maturation to SP thymocytes. Elimination of Bclx by gene targeting dramatically shortens the survival of DP thymocytes but not the survival of SP thymocytes or peripheral SP T cells, These data suggest that the induction of Bclx during thymic maturation plays a critical role in regulating the length of time DP thymocytes survive in the absence of selection.