Expression of vanilloid receptor subtype 1 in cutaneous sensory nerve fibers, mast cells, and epithelial cells of appendage structures
Expression of vanilloid receptor subtype 1 in cutaneous sensory nerve fibers, mast cells, and epithelial cells of appendage structures
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DOI：
10.1111/j.0906-6705.2004.0178.x
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发表时间：
2004-03-01
影响因子：
3.6
通讯作者：
Steinhoff, M
中科院分区：
文献类型：
--
作者：
Ständer, S;Moormann, C;Steinhoff, M
结合辣椒素的香草素受体亚型1（VR 1）/（TRPV 1）是一种非选择性阳离子通道，最近在体外和体内的人类角质形成细胞中得到了证实。然而，VR 1定位在其他皮肤隔间，特别是皮肤神经纤维的描述仍然缺乏。因此，我们研究了一系列（n = 26）正常（n = 7），患病（n = 13）[结节性黑色素瘤（PN）（n = 10），全身瘙痒症（n = 1），肥大细胞增多症（n = 2）]，辣椒素治疗的人皮肤（n = 6）的VR 1免疫反应性以及mRNA和蛋白质表达。在皮肤感觉神经纤维、肥大细胞、表皮角质形成细胞、真皮血管、毛囊内根片和漏斗部、分化的皮脂腺细胞、汗腺导管和外泌汗腺分泌部均可观察到VR 1免疫反应。经逆转录聚合酶链反应和蛋白质印迹分析，VR 1检测肥大细胞和角质形成细胞从人类皮肤。在PN的皮炎皮肤中，表皮角质形成细胞和神经纤维中的VR 1表达高度增加，在辣椒素应用后恢复正常。在辣椒素治疗期间，观察到神经肽（P物质，降钙素基因相关肽）减少。停止辣椒素治疗后，神经肽在皮肤神经中重新积聚。总之，VR 1广泛分布在皮肤中，表明该受体在例如伤害感受和神经源性炎症中起主要作用。
The vanilloid receptor subtype 1 (VR1)/(TRPV1), binding capsaicin, is a non-selective cation channel that recently has been shown in human keratinocytes in vitro and in vivo. However, a description of VR1 localization in other cutaneous compartments in particular cutaneous nerve fibers is still lacking. We therefore investigated VR1 immunoreactivity as well as mRNA and protein expression in a series (n = 26) of normal (n = 7), diseased (n = 13) [prurigo nodularis (PN) (n = 10), generalized pruritus (n = 1), and mastocytosis (n = 2)], and capsaicin-treated human skin (n = 6). VR1 immunoreactivity could be observed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands. Upon reverse transcriptase-polymerase chain reaction and Western blot analysis, VR1 was detected in mast cells and keratinocytes from human skin. In pruritic skin of PN, VR1 expression was highly increased in epidermal keratinocytes and nerve fibers, which was normalized after capsaicin application. During capsaicin therapy, a reduction of neuropeptides (substance P, calcitonin gene-related peptide) was observed. After cessation of capsaicin therapy, neuropeptides re-accumulated in skin nerves. In conclusion, VR1 is widely distributed in the skin, suggesting a major role for this receptor, e.g. in nociception and neurogenic inflammation.