Nociceptin differentially affects morphine-induced dopamine release from the nucleus accumbens and nucleus caudate in rats
Nociceptin differentially affects morphine-induced dopamine release from the nucleus accumbens and nucleus caudate in rats
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DOI：
10.1016/s0196-9781(00)00250-3
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发表时间：
2000-07-01
期刊：
影响因子：
3
通讯作者：
Pieretti, S
中科院分区：
文献类型：
--
作者：
Di Giannuario, A;Pieretti, S
用微透析电化学检测法研究了痛敏素对吗啡诱导的大鼠延髓核和尾状核多巴胺（DA）、3，4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）释放的影响。脑室内（i. c. v.）在剂量为2、5和10 nmol/大鼠时，既不改变多巴胺也不改变尾状核壳或尾状核中代谢物的释放。腹膜内（i. p.）（2，5，和10毫克/公斤）增加DA和代谢产物释放更多的壳中的丘脑核比在尾状核。当在吗啡（5或10 mg/kg）给药前15分钟给予痛敏素（5或10 nmol）时，它显著减少吗啡诱导的多巴胺和代谢产物在背侧核壳中的释放，而在尾状核中只观察到轻微的、不显著的减少。我们的数据表明，nociceptin可能调节与吗啡诱导的DA释放更多的刺激作用在延髓核比在尾状核，并与最近的观察结果，nociceptin逆转乙醇和吗啡诱导的条件性位置偏爱是一致的。因此，孤啡肽诱导的多巴胺释放减少吗啡刺激的延髓核，和孤啡肽在条件性位置偏爱程序中获得的结果表明，孤啡肽在滥用药物的行为和神经化学作用的调制中的作用。(C)2000 Elsevier Science Inc. All rights reserved.
The effects induced by nociceptin on morphine-induced release of dopamine (DA), 3,4-dihydroxyphenilacetic acid (DOPAC) and homovanillic acid (HVA) in the nucleus accumbens and nucleus caudate were studied in rats by microdialysis with electrochemical detection. Nociceptin administered intracerebroventricularly (i.c.v.) at doses of 2, 5 and 10 nmol/rat changed neither DA nor metabolites release in the shell of the nucleus accumbens or in the nucleus caudate. Morphine administered intraperitoneally (i.p.) (2, 5, and 10 mg/kg) increased DA and metabolites release more in the shell of the nucleus accumbens than in the nucleus caudate. When nociceptin (5 or 10 nmol) was administered 15 min before morphine (5 or 10 mg/kg), it significantly reduced morphine-induced DA and metabolites release in the shell of the nucleus accumbens, whereas only a slight, nonsignificant reduction was observed in the nucleus caudate. Our data indicate that nociceptin may regulate the stimulating action associated with morphine-induced DA release more in the nucleus accumbens than in the nucleus caudate, and are consistent with recent observations that nociceptin reversed ethanol- and morphine-induced conditioned place preference. Therefore, the nociceptin-induced reduction of DA release stimulated by morphine in the nucleus accumbens, and the results obtained with nociceptin in the conditioned place preference procedure suggest a role for nociceptin in the modulation of the behavioral and neurochemical effects of abuse drugs. (C) 2000 Elsevier Science Inc. All rights reserved.