The G protein-coupled receptor GPRC5B contributes to neurogenesis in the developing mouse neocortex
The G protein-coupled receptor GPRC5B contributes to neurogenesis in the developing mouse neocortex
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DOI：
10.1242/dev.099754
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发表时间：
2013-11-01
期刊：
影响因子：
4.6
通讯作者：
Sanada, Kamon
中科院分区：
文献类型：
--
作者：
Kurabayashi, Nobuhiro;Minh Dang Nguyen;Sanada, Kamon
发育中的大脑中的神经祖细胞产生神经元和神经胶质。多种外源性信号分子及其同源膜受体已被确定控制神经祖细胞的命运。然而，G蛋白偶联受体在大脑细胞命运决定中的作用在很大程度上仍然是假定的。在这里，我们发现编码孤儿G蛋白偶联受体的GPRC5B存在于小鼠发育中的新皮层皮层祖细胞的心室表面，并且是其神经元分化所必需的。缺乏gprc5b的祖细胞不能接受神经元的命运，最终成为星形胶质细胞。此外，gprc5b介导的信号传导与β -连环蛋白信号传导的适当调节有关，β -连环蛋白信号传导是决定祖细胞命运的关键途径。我们的研究揭示了G蛋白偶联受体信号在皮质祖细胞神经元命运决定中的作用。
Neural progenitor cells in the developing brain give rise to neurons and glia. Multiple extrinsic signalling molecules and their cognate membrane receptors have been identified to control neural progenitor fate. However, a role for G protein-coupled receptors in cell fate decisions in the brain remains largely putative. Here we show that GPRC5B, which encodes an orphan G protein-coupled receptor, is present in the ventricular surface of cortical progenitors in the mouse developing neocortex and is required for their neuronal differentiation. GPRC5B-depleted progenitors fail to adopt a neuronal fate and ultimately become astrocytes. Furthermore, GPRC5B-mediated signalling is associated with the proper regulation of beta-catenin signalling, a pathway crucial for progenitor fate decision. Our study uncovers G protein-coupled receptor signalling in the neuronal fate determination of cortical progenitors.