Induction of neutralizing antibodies to papillomaviruses by anti-idiotypic antibodies.
Induction of neutralizing antibodies to papillomaviruses by anti-idiotypic antibodies.
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通过抗独特型抗体诱导针对乳头瘤病毒的中和抗体。
DOI：
10.1006/viro.1995.1346
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Cladel,NM
中科院分区：
文献类型：
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作者：
Christensen,ND;Reed,CA;Cladel,NM
抗独特型抗体（抗-Ids）针对三种小鼠单克隆抗体（MAbs），中和三种不同的乳头瘤病毒。中和单克隆抗体（N-MAbs）产生的感染性人乳头瘤病毒11型（HPV-11），棉尾兔乳头瘤病毒（CRPV），和牛乳头瘤病毒1型（BPV-1），和所有识别的表面构象表位的病毒粒子变性后丢失。使用一组10种N-MAb（4种HPV-11中和、5种CRPV中和和1种BPV-1中和）在ELISA中筛选多克隆抗Ids，并观察到交叉反应性独特型反应性。亲和纯化的抗H11.B2（HPV-11 N-MAb）与其他3种HPV-11 N-MAb交叉反应，与非中和性HPV-11 MAb和所有5种CRPV N-MAb不反应，但令人惊讶的是与BPV-1 N-MAb反应。相反，亲和纯化的抗CRPV-1A（CRPV N-MAb）与所有5种CRPV N-MAb反应，但不与任何其他MAb反应。亲和纯化的抗B1.A1（BPV-1 N-MAb）也显示出与4种HPV-11 N-MAb的意外交叉反应性，但与任何CRPV N-MAb均无交叉反应性。三种多克隆兔抗Ids中的两种在免疫后的BALB/c小鼠中诱导强烈的抗活体抗体应答（包括病毒中和抗体）。结果表明，在小鼠品系内，乳头瘤病毒中和抗体共享交叉反应独特位，将N-MAb分为几个子集。一组包括CRPV N-MAb，另一组包括针对明显无关的乳头瘤病毒的N-MAb，包括HPV-11、BPV-1和可能的HPV-6。
Anti-idiotypic antibodies (anti-Ids) were generated against three mouse monoclonal antibodies (MAbs) which neutralized three different papillomaviruses. The neutralizing MAbs (N-MAbs) were generated against infectious human papillomavirus type 11 (HPV-11), cottontail rabbit papillomavirus (CRPV), and bovine papillomavirus type 1 (BPV-1), and all recognized surface conformational epitopes that were lost upon denaturation of the virions. The polyclonal anti-Ids were screened in an ELISA using a panel of 10 N-MAbs (4 HPV-11 neutralizing, 5 CRPV neutralizing, and 1 BPV-1 neutralizing), and cross-reactive idiotypic reactivity was observed. Affinity-purified anti-H11.B2 (HPV-11 N-MAb) cross-reacted with the 3 other HPV-11 N-MAbs, was unreactive to a nonneutralizing HPV-11 MAb and all 5 CRPV N-MAbs, but surprisingly was reactive to the BPV-1 N-MAb. In contrast, affinity-purified anti-CRPV-1A (CRPV N-MAb) reacted with all 5 CRPV N-MAbs, but not with any other MAb. Affinity-purified anti-B1.A1 (BPV-1 N-MAb) also showed unexpected cross-reactivity with the 4 HPV-11 N-MAbs, but not with any of the CRPV N-MAbs. Two of the three polyclonal rabbit anti-Ids induced strong anti-vital antibody responses (including virus-neutralizing antibodies) in BALB/c mice following immunization. The results indicate that within a mouse strain, papillomavirus-neutralizing antibodies share cross-reactive idiotopes that group the N-MAbs into several subsets. One group includes the CRPV N-MAbs, and another group includes N-MAbs directed to apparently unrelated papillomaviruses, including HPV-11, BPV-1, and possibly HPV-6.