Effect of post-drawing and tension on enzymatic degradation of electrospun polycaprolactone nanofibers
Effect of post-drawing and tension on enzymatic degradation of electrospun polycaprolactone nanofibers
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DOI：
10.1016/j.mtcomm.2022.104990
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发表时间：
2022-12-01
影响因子：
3.8
通讯作者：
Beachley, Vince
中科院分区：
文献类型：
--
作者：
Brennan, David A.;Flamini, Matthew D.;Beachley, Vince
电纺聚己内酯纳米纤维的酶降解，以评估后拉伸处理和张力对降解速率的影响。将电纺聚己内酯（PCL）纳米纤维拉伸至增加的长度，然后浸没在假单胞菌脂肪酶（PS脂肪酶）溶液中7天。通过第0、3和7天的质量、拉伸强度、结晶度百分比、分子量和大分子链排列的变化评价降解过程和劣化程度。降解速率取决于纤维的结晶度百分比以及纤维的结晶和非晶部分的排列程度。拉伸后的PCL纤维在PS脂肪酶中保持质量和拉伸强度超过7天，而未拉伸的纤维在1天内降解。酶解前纤维的预张力对保持降解过程中的大分子结构和拉伸强度至关重要。质量和机械强度损失而分子量没有降低，表明降解是通过材料随时间的表面侵蚀而发生的，而不是酶渗透和本体降解。结晶度和链排列的测量说明了自动化轨道绘制对晶体生长和链排列的进展的影响，以及在多日降解期间发生的大分子结构的变化。
Electrospun polycaprolactone nanofibers were enzymatically degraded to evaluate the effect of post-draw processing and tension on the rate of degradation. Electrospun polycaprolactone (PCL) nanofibers were drawn to increasing lengths, then submerged in pseudomonas lipase (PS Lipase) solution for a 7-day period. The degradation process and extent of deterioration were evaluated by changes in mass, tensile strength, percent crystallinity, molecular weight, and macromolecular chain alignment on day 0, 3, and 7. The rate of degradation was dependent on the percent crystallinity of the fiber and the degree of alignment in crystalline and amorphous portions of the fiber. Post-drawn PCL fibers maintained mass and tensile strength over 7 days in PS Lipase, while undrawn fibers, degraded within 1 day. Pretension in fibers before enzymatic incubation was critical to maintain the macromolecular structure and tensile strength over the degradation period. Loss of mass and mechanical strength without molecular weight reduction indicated that degradation occured via surface erosion of the material over time rather than enzyme penetration and bulk degradation. Measurement of crystallinity and chain alignment illustrated the effect of automated track drawing on the progression of crystal growth and chain alignment, as well as the changes in macromolecular structure which occurred during the multi-day degradation period.