Novel translationally controlled tumor protein homologue in the buccal gland secretion of Lampetra japonica
Novel translationally controlled tumor protein homologue in the buccal gland secretion of Lampetra japonica
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日本七灯颊腺分泌中新型翻译控制肿瘤蛋白同源物
DOI：
10.1016/j.biochi.2008.08.002
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发表时间：
2008-11-01
期刊：
影响因子：
3.9
通讯作者：
Li, Qingwei
中科院分区：
文献类型：
--
作者：
Sun, Jing;Wu, Yu;Li, Qingwei
根据cDNA文库的信息和表达序列标签的初步分析，我们从日本七鳃鳗（Lampetra japonica）的颊腺中克隆了一个与肿瘤抑制蛋白（TCTP）同源的基因。序列分析表明，L. Japonica TCTP序列分析表明，该基因具有两个高度同源的特征区域，分别命名为TCTP-1和TCTP-2。TCTP在进化中高度保守。与影响宿主免疫应答的寄生虫TCTPs的识别相似度在40%以上。31个TCTP序列的系统发育分析表明七鳃鳗与有颌类脊椎动物的亲缘关系比文昌鱼更近，是有颌类的姐妹类群。L.的TCTP基因。在pET 23 b载体中表达，并通过使用His Bind亲和层析纯化。在新西兰兔体内制备了抗重组蛋白的多克隆抗体。对重组蛋白在口腔腺分泌物中的定位的免疫印迹分析证明，重组TCTP是一种分泌蛋白，其可能通过非经典分泌途径分泌。对重组TCTP进行了体外释放组胺的研究。它介导大鼠嗜碱性白血病（RBL-2 H3）细胞释放组胺。TCTP通过调节肥大细胞、嗜碱性粒细胞、嗜酸性粒细胞以及T和B淋巴细胞的细胞因子分泌来连接先天性和适应性免疫应答。提示TCTP可能参与L. japonica和寄主。(c)2008年，Elsevier Masson SAS。All rights reserved.
We have cloned a homologue of the translationally controlled tumor protein (TCTP) from the buccal gland of Lampetra japonica according to information from a cDNA library and primary analysis of expressed sequence tags. Sequence analysis of L. japonica TCTP showed that it had two signature regions of high sequence homology termed TCTP-1 and TCTP-2, respectively. TCTP is highly conserved in evolution. It showed more than 40% identification similarities with parasite TCTPs that had effect on immune responses of host. Phylogeny of 31 TCTP sequences showed that lamprey was closer to jawed vertebrates than to Amphioxus and was a sister group of gnathostomes. TCTP gene from L. japonica was expressed in a pET23b vector and purified by using His Bind affinity chromatography. Polyclonal antibody to recombinant protein was generated in New Zealand Rabbit. Immunoblot analysis to localize the recombinant protein in buccal gland secretion proves that recombinant TCTP is a secretion protein, which may be secreted through a non-classical secretion pathway. A characterization study shows that recombinant TCTP has histamine-releasing function in vitro. It mediated histamine release from rat basophilic leukemia (RBL-2H3) cells. TCTP links both the innate and the adaptive immune responses by modulating the secretion of cytokines from mast cells, basophils, eosinophils, and T and B lymphocytes. These may indicate a potential role of TCTP in the inflammatory process and immune regulation between L. japonica and host. (c) 2008 Elsevier Masson SAS. All rights reserved.