CARRIER-MEDIATED UPTAKE OF GLYPHOSATE IN BROAD BEAN (VICIA-FABA) VIA A PHOSPHATE TRANSPORTER
CARRIER-MEDIATED UPTAKE OF GLYPHOSATE IN BROAD BEAN (VICIA-FABA) VIA A PHOSPHATE TRANSPORTER
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DOI：
10.1111/j.1399-3054.1993.tb02508.x
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发表时间：
1993-04-01
影响因子：
6.4
通讯作者：
DELROT, S
中科院分区：
文献类型：
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作者：
DENIS, MH;DELROT, S
使用蚕豆叶（Vicia faba L.）的原生质体研究了除草剂草甘膦（N-膦酰甲基甘氨酸）可能通过质膜的磷酸盐转运蛋白被植物细胞吸收的可能性。膦甲酸是动物细胞中磷酸盐转运的强大抑制剂，首次被证明是蚕豆原生质体中磷酸盐摄取的竞争性抑制剂。草甘膦能够抑制原生质体对磷酸盐的吸收，并部分保护磷酸盐转运蛋白免受膦甲酸的抑制。浓度依赖性研究表明，草甘膦吸收在低草甘膦浓度（0.5至3μM）下表现出饱和阶段，并在较高浓度（高达100μM）下呈现线性吸收。对氯汞苯磺酸、叠氮化钠和羰基氰化物间氯苯腙对草甘膦吸收的抑制作用在 1 浓度时比 100 μM 草甘膦浓度强得多。动力学表明，草甘膦转运的可饱和成分被磷酸盐或膦甲酸竞争性抑制。结论是草甘膦可以通过质膜的磷酸盐转运蛋白被吸收。
The possibility that the herbicide glyphosate (N-phosphonomethylglycine) may be taken up in plant cells via a phosphate transporter of the plasma membrane was investigated using protoplasts of broad bean leaves (Vicia faba L.). Phosphonoformic acid, a powerful inhibitor of phosphate transport in animal cells, was first demonstrated to be a competitive inhibitor of phosphate uptake in broad bean protoplasts. Glyphosate was able to inhibit phosphate uptake into the protoplasts, and to protect partially the phosphate transporter from inhibition by phosphonoformic acid. Concentration dependence studies showed that glyphosate uptake exhibited a saturable phase at low glyphosate concentrations (0.5 to 3 muM), superimposed by a linear uptake at higher concentrations (up to 100 muM). Inhibition of glyphosate uptake by para-chloromercuribenzene sulphonic acid, sodium azide and carbonyl-cyanide-m-chlorophenylhydrazone was much stronger at 1 than at 100 muM glyphosate. Kinetics indicated that the saturable component of glyphosate transport was competitively inhibited by either phosphate or phosphonoformic acid. It is concluded that glyphosate can be absorbed via a phosphate transporter of the plasma membrane.