Biotin-containing intranuclear inclusions in endometrial glands during gestation and puerperium.
Biotin-containing intranuclear inclusions in endometrial glands during gestation and puerperium.
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妊娠和产褥期子宫内膜腺体中含有生物素的核内包涵体。
DOI：
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发表时间：
1993
影响因子：
3.5
通讯作者：
A. Moriuchi
中科院分区：
文献类型：
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作者：
S. Yokoyama;K. Kashima;S. Inoue;T. Daa;I. Nakayama;A. Moriuchi
有时在子宫内膜腺体中观察到核内包涵体，尤其是在产褥期，表面上它们类似于疱疹病毒感染的细胞。为了研究内含物的意义，检查了215例受试者的子宫内膜和胎盘组织，其中包括69例妊娠相关样本。单独用过氧化物酶标记的抗生物素蛋白进行筛选，然后进行过氧化物酶反应，只有妊娠相关的子宫内膜（32例）表现出核内包涵体的反应性。使用过氧化物酶-抗过氧化物酶方法，除六例外，所有病例的这些核内内含物均显示生物素免疫染色阳性，而任何病例均未出现单纯疱疹反应。32例子宫内膜组织取自妊娠第16周至产后第37天。虽然这些核内包涵体的组织发生尚未明确，但由于核内生物素和抗生物素蛋白-生物素-过氧化物酶复合物法中的假阳性组织化学反应，它们不应被误认为是疱疹病毒感染的子宫内膜细胞中的包涵体。
Intranuclear inclusions sometimes are observed in endometrial glands, especially during puerperium, and superficially they resemble those from herpesvirus-infected cells. To study the significance of the inclusions, endometrial and placental tissue from 215 subjects, including 69 pregnancy-associated samples, were examined. With screening by peroxidase-labeled avidin alone, followed by the peroxidase reaction, only pregnancy-related endometrium (in 32 cases) demonstrated reactivity in the intranuclear inclusions. These intranuclear inclusions demonstrated positive immunostaining for biotin using the peroxidase-antiperoxidase method in all but six cases, whereas there was no reaction for Herpes simplex in any of the cases. The endometrial tissues of the 32 cases were obtained from the 16th gestational week to the 37th postpartum day. Although the histogenesis of these intranuclear inclusions has not been clarified, they should not be mistaken for those in endometrial cells infected by herpesvirus because of intranuclear biotin and a false-positive histochemical reaction in the avidin-biotin-peroxidase complex method.