Role of Ppt1 in multiple stress responses in Candida albicans
Role of Ppt1 in multiple stress responses in Candida albicans
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Ppt1 在白色念珠菌多重应激反应中的作用
DOI：
10.1007/s11434-014-0552-7
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发表时间：
2014-11-01
影响因子：
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通讯作者：
Sang, Jianli
中科院分区：
文献类型：
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作者：
Hu, Kangdi;Li, Wanjie;Sang, Jianli
为了研究CaPpt 1的功能，我们从白色念珠菌基因组中删除了PPT 1基因，依次用选择性标记ARG 4和HIS 1替换整个编码区。结果表明，Ppt 1基因的缺失对菌丝的形成没有影响。白念珠菌在血清诱导下，并引起增强的敏感性DNA损伤，Calcofluor白色和盐诱导的压力。我们还发现，Ppt 1是不需要的基因毒素，甲基甲烷磺酸盐和羟基脲处理的细胞的表型反应。流式细胞仪分析表明，ppt 1 Δ细胞和野生型细胞在DNA损伤应激时表现出相似的G2/M期阻滞特征。Ppt 1不是DNA损伤反应途径激活所必需的，如在DNA损伤应激下Ppt 1 Δ细胞中Rad 53和Rfa 2的正常磷酸化所示。我们认为Ppt 1在C.白色念珠菌
To study the function of CaPpt1, we deleted PPT1 gene from the Candida albicans genome by sequentially replacing the entire coding region with the selectable markers ARG4 and HIS1. The results showed that the deletion of Ppt1 did not affect the hyphal formation of C. albicans under serum induction and caused enhanced sensitivity to DNA damage, Calcofluor white and salt-induced stress. We also found that Ppt1 was not required for the phenotypic response of cells treated with the genotoxins, methylmethane sulfonate and hydroxyurea. Flow cytometric analyses indicated that ppt1Δ cells and wild-type cells showed similar G2/M arrest profiles when exposed to DNA damage stress. Ppt1 was not required for the activation of the DNA damage response pathway, as indicated by normal phosphorylation of Rad53 and Rfa2 in ppt1Δ cells under DNA damage stress. We suggest that Ppt1 plays important roles in response to various stress conditions in C. albicans.