Enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection using urine samples and its application in Bangladesh
Enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection using urine samples and its application in Bangladesh
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尿样酶联免疫吸附法诊断班克罗夫蒂菌感染及其在孟加拉国的应用
DOI：
10.1016/j.parint.2013.08.007
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发表时间：
2013
期刊：
影响因子：
1.9
通讯作者：
Kimura E
中科院分区：
文献类型：
--
作者：
Samad MS;Itoh M;Moji K;Hossain M;Mondal D;Alam MS;Kimura E
在斯里兰卡，尿液ELISA在检测丝虫特异性IgG 4方面显示出较高的灵敏度和特异性。在对幼儿的流行率研究中，它的阳性率也比抗原检测高得多。在这项研究中，我们证实了尿液ELISA在孟加拉国领域的实用性。ELISA检测出89（85%）的105个ICT抗原试验阳性的流行区。ICT和微丝蚴同时阳性的敏感性为97%（30/31）。非流行区104例ICT阴性者ELISA均为阴性（特异性100%）。在一项患病率研究中，319名来自低流行区的幼儿（5-10岁）在5轮MDA后，7名（2.2%）被检测到目前的尿液检测，但只有1名（0.3%）ICT（P = 0.075）。令人满意的高灵敏度，100%的特异性和有效的病例检测年轻的年龄沿着与范围分析滴度将表明尿液ELISA是一个有效的工具，在后MDA调查，以确认消除或检测在孟加拉国的复苏。
In Sri Lanka, urine ELISA showed high sensitivity and specificity in detecting filaria-specific IgG4. It also produced much higher positive rates than antigen tests in prevalence studies with young children. In this study, we have confirmed the usefulness of urine ELISA in the field of Bangladesh. The ELISA detected 89 of 105 (85%) ICT antigen test positive subjects in endemic areas. With both ICT and microfilaria positives, the sensitivity was 97% (30/31). All of 104 ICT negative people in a non-endemic area were ELISA negative (100% specificity). In a prevalence study with 319 young children (5–10 years) from a low endemic area after five rounds of MDA, seven (2.2%) were detected by the present urine test, but only one (0.3%) by ICT (P = 0.075). The satisfactorily high sensitivity, 100% specificity and effective case detection among young ages along with scope for analyzing the titers will indicate urine ELISA to be an effective tool in the post-MDA surveys to confirm elimination or to detect resurgence in Bangladesh.