In vivo magnetic resonance imaging of dendritic cell migration into the draining lymph nodes of mice
In vivo magnetic resonance imaging of dendritic cell migration into the draining lymph nodes of mice
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DOI：
10.1002/eji.200535742
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发表时间：
2006-09-01
影响因子：
5.4
通讯作者：
Lutz, Manfred B.
中科院分区：
文献类型：
--
作者：
Baumjohann, Dirk;Hess, Andreas;Lutz, Manfred B.
树突状细胞(DC)向引流淋巴结的迁移是T细胞启动的关键。在这里，我们展示了磁共振成像(MRI)可以用来可视化DC在体内的迁移。我们将临床批准的小颗粒氧化铁(SPIO)与硫酸鱼精蛋白相结合，以实现小鼠骨髓来源DC的有效摄取。SPIO-DC基本上没有改变，注射到小鼠的足垫后，它们迁移到引流淋巴结的T细胞区，这可以通过MRI观察到。铁染色和免疫组织学证实，明显的MRI信号减少模式与SPIO-DC的检测主要在Thy-1.2(+)B220(-)T细胞区相关。在高分辨率下，1×10(6)注入的SPIO-DC仍可观察到清晰的信号减少模式，导致检测到约2000 DC。对照注射CCR7(-/-)小鼠来源的归巢不能的SPIO-DC或单独注射SPIO不能到达T细胞区。综上所述，结果表明，临床批准的造影剂可以在体内以高分辨率的MRI无创性地显示DC向引流淋巴结的迁移。因此，该方案还允许对免疫反应进行动态成像，并基于MRI跟踪患者中的人类DC。
Dendritic cell (DC) migration into the draining lymph nodes is critical for T cell priming. Here, we show that magnetic resonance imaging (MRI) can be used to visualize DC migration in vivo. We combined clinically approved small particles of iron oxide (SPIO) with protamine sulfate to achieve efficient uptake by murine bone marrow-derived DC. SPIO-DC were largely unaltered and after injection into the footpads of mice, they migrated into the T cell areas of the draining lymph nodes, which could be visualized by MRI. Distinct MRI signal reduction patterns correlated with the detection of SPIO-DC mainly within Thy-1.2(+) B220(-) T cell areas, as confirmed by iron staining and immunohistology. Clear signal reduction patterns could still be observed with 1 x 10(6) injected SPIO-DC at high resolution, resulting in the detection of about 2000 DC. Control injections of homing-incompetent SPIO-DC derived from CCR7(-/-) mice or SPIO alone did not reach the T cell areas. Taken together, the results demonstrate that clinically approved contrast agents allow the non-invasive visualization of DC migration into the draining lymph node by MRI in vivo at high resolution. This protocol therefore also allows dynamic imaging of immune responses and MRI-based tracking of human DC in patients.