Human nucleolar protein Nop52 (RRP1/NNP-1) is involved in site 2 cleavage in internal transcribed spacer 1 of pre-rRNAs at early stages of ribosome biogenesis.
Human nucleolar protein Nop52 (RRP1/NNP-1) is involved in site 2 cleavage in internal transcribed spacer 1 of pre-rRNAs at early stages of ribosome biogenesis.
复制标题
DOI：
10.1093/nar/gkv470
复制
发表时间：
2015-06-23
影响因子：
14.9
通讯作者：
Takahashi N
中科院分区：
文献类型：
--
作者：
Yoshikawa H;Ishikawa H;Izumikawa K;Miura Y;Hayano T;Isobe T;Simpson RJ;Takahashi N
在哺乳动物核糖体生物发生的早期阶段，两个核糖体亚基40 S和60 S通过将大的90 S前核糖体颗粒（90 S）分裂成前40 S和前60 S前核糖体颗粒（前40 S和前60 S）而产生。我们以前提出，Nop 52（RRP 1/NNP-1）取代原纤维蛋白与p32（C1 QBP）的结合是驱动这一分裂过程的关键事件。然而，RRP 1的替代如何与前rRNA的内切和/或外切核糖核酸裂解偶联仍然未知。在这项研究中，我们证明，RRP 1缺陷抑制ITS 1的47 S/45 S，41 S和36 S前体rRNA在人类细胞中的位点2切割。RRP 1也存在于90 S中，并依赖于活性RNA聚合酶I转录定位于核仁的致密纤维成分中。此外，XRN 2和RRP 1的双敲低揭示RRP 1加速47 S、45 S和41 S前体rRNA的位点2切割。这些数据表明，RRP 1不仅参与与90 S上的C1 QBP竞争性结合，而且还参与在人核糖体生物发生的早期阶段前体rRNA的ITS 1中的位点2切割;因此，很可能RRP 1将位点2的切割与90 S的物理分裂整合为前40 S和前60 S。
During the early steps of ribosome biogenesis in mammals, the two ribosomal subunits 40S and 60S are produced via splitting of the large 90S pre-ribosomal particle (90S) into pre-40S and pre-60S pre-ribosomal particles (pre-40S and pre-60S). We previously proposed that replacement of fibrillarin by Nop52 (RRP1/NNP-1) for the binding to p32 (C1QBP) is a key event that drives this splitting process. However, how the replacement by RRP1 is coupled with the endo- and/or exo-ribonucleolytic cleavage of pre-rRNA remains unknown. In this study, we demonstrate that RRP1 deficiency suppressed site 2 cleavage on ITS1 of 47S/45S, 41S and 36S pre-rRNAs in human cells. RRP1 was also present in 90S and was localized in the dense fibrillar component of the nucleolus dependently on active RNA polymerase I transcription. In addition, double knockdown of XRN2 and RRP1 revealed that RRP1 accelerated the site 2 cleavage of 47S, 45S and 41S pre-rRNAs. These data suggest that RRP1 is involved not only in competitive binding with fibrillarin to C1QBP on 90S but also in site 2 cleavage in ITS1 of pre-rRNAs at early stages of human ribosome biogenesis; thus, it is likely that RRP1 integrates the cleavage of site 2 with the physical split of 90S into pre-40S and pre-60S.
登录
查看更多内容
影响因子：
14.9
作者：
Preti M;O'Donohue MF;Montel-Lehry N;Bortolin-Cavaillé ML;Choesmel V;Gleizes PE
通讯作者：
Gleizes PE
DOI：
10.1083/jcb.200501141
发表时间：
2005-08-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hölzel M;Rohrmoser M;Schlee M;Grimm T;Harasim T;Malamoussi A;Gruber-Eber A;Kremmer E;Hiddemann W;Bornkamm GW;Eick D
通讯作者：
Eick D
影响因子：
16
作者：
Lapik, YR;Fernandes, CJ;Pestov, DG
通讯作者：
Pestov, DG
影响因子：
3.3
作者：
Dousset, T;Wang, C;Huang, S
通讯作者：
Huang, S
影响因子：
14.9
作者：
Grimm T;Hölzel M;Rohrmoser M;Harasim T;Malamoussi A;Gruber-Eber A;Kremmer E;Eick D
通讯作者：
Eick D