Establishment of mouse lymphokine-activated killer cell clones and their properties.
Establishment of mouse lymphokine-activated killer cell clones and their properties.
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DOI：
10.1111/j.1349-7006.1991.tb01870.x
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发表时间：
1991-04
期刊：
影响因子：
--
通讯作者：
Hashimoto Y
中科院分区：
文献类型：
--
作者：
Kato K;Sato N;Tanabe T;Yagita H;Agatsuma T;Hashimoto Y
为了评估淋巴因子激活杀伤细胞（LAK）的特性，我们从C57BL/6小鼠脾细胞诱导的LAK细胞系中建立了小鼠LAK细胞克隆。虽然这些克隆表达与亲本LAK细胞相似的表型，但Lyt‐2在克隆的有限部分中表达。所有克隆均在细胞表面表达T3 CD3和T细胞受体(TcR) αβ。用c - β1和j - β探针测定了来自同一亲本细胞系的无性系的TcR重排模式相同，而来自不同细胞系的无性系的TcR重排模式不同。利用针对淋巴细胞功能相关抗原1 （LFA - 1）的单克隆抗体（mAb）检测LAK靶细胞结合的分子。该单抗（称为KBA）对LAK靶细胞结合和LAK细胞克隆的细胞溶解活性均有抑制作用，表明LFA - 1在LAK细胞克隆中的主要作用。LAK细胞克隆中perform mRNA的表达量与其细胞溶解活性无关。
To assess the properties of lymphokine‐activated killer (LAK) cells, we established mouse LAK cell clones from LAK cell lines induced from C57BL/6 mouse spleen cells. Although these clones expressed similar phenotypes to the parent LAK cells, Lyt‐2 was expressed in a restricted portion of the clones. All clones were found to express T3 CD3 and T cell receptor (TcR) αβ on their cell surface. Rearrangement patterns of TcR were the same among the clones derived from the same parent cell line but differed in those from different cell lines as determined by using Cβ1 and Jβ probes. The molecules responsible for LAK‐target cell binding were examined by using a monoclonal antibody (mAb) against lymphocyte function associated antigen 1 (LFA‐1). This mAb (termed KBA) showed inhibitory effects on both LAK‐target cell binding and cytolytic activity of LAK cell clones, indicating a principal role of LFA‐1 in LAK cell clones. The magnitude of perform mRNA expression in LAK cell clones was unrelated to their cytolytic activities.