Arginine induces protein self-assembly into nanofibers for triggering osteogenic differentiation of stem cells
Arginine induces protein self-assembly into nanofibers for triggering osteogenic differentiation of stem cells
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精氨酸诱导蛋白质自组装成纳米纤维，从而触发干细胞的成骨分化
DOI：
10.1039/d1tb01921j
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发表时间：
2021
影响因子：
7
通讯作者：
Yang Mingying
中科院分区：
文献类型：
--
作者：
Lei Fang;Zhou Guanshan;Chen Yuping;Cai Jiangfeng;Wang Jie;Shuai Yajun;Xu Zongpu;Wang Zhangfu;Mao Chuanbin;Yang Mingying
尽管蚕丝蛋白被认为在构建组织工程支架方面很有前途，但蚕丝蛋白之一家蚕丝胶蛋白（BS）在制造纳米纤维支架方面的加工能力有限，因为其表面电荷的各向异性反而促进了凝胶化。为了克服这一艰巨的挑战，我们开发了一种温和而简单的方法，将BS组装成纳米纤维和纳米纤维支架。简单地说，将精氨酸加入到BS水溶液中，使BS的负电荷减少，从而诱导BS在溶液中自组装成纳米纤维。圆二色性（CD）和傅里叶变换红外光谱（FT-IR）表明，精氨酸促进了BS中β-薄片构象的形成，提高了BS的热稳定性。此外，精氨酸诱导的BS纳米纤维溶液可以浇铸成丰富的网状纳米纤维结构的支架。在培养基中不添加分化诱导剂的情况下，BS支架可促进骨髓间充质干细胞（BMSCs）的粘附和生长，促进其成骨分化。我们的研究提出了一种将蛋白质组装成成骨纳米纤维支架以诱导再生医学中的干细胞分化的新策略。
Although silk proteins are considered promising in building a scaffold for tissue engineering, one of the silk proteins, Bombyx mori silk sericin (BS), has limited processability in producing nanofibrous scaffolds because its surface charge anisotropy promotes gelation instead. To overcome this daunting challenge, we developed a mild and simple procedure for assembling BS into nanofibers and nanofibrous scaffolds. Briefly, arginine was added to the aqueous BS solution to reduce the negative charge of BS, thereby inducing BS to self-assemble into nanofibers in the solution. Circular dichroism (CD) and Fourier transform infrared (FT-IR) spectra showed that arginine promoted the formation of β-sheet conformation in BS and increased its thermal stability. Furthermore, the arginine-induced BS nanofiber solution could be casted into scaffolds made of abundant network-like nanofibrous structures. The BS scaffolds promoted cell adhesion and growth and stimulated osteogenic differentiation of the bone marrow mesenchymal stem cells (BMSCs) in the absence of differentiation inducers in culture media. Our study presents a new strategy for assembling proteins into osteogenic nanofibrous scaffolds for inducing stem cell differentiation in regenerative medicine.