Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy
Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy
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DOI：
10.1016/j.susc.2005.12.058
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发表时间：
2006-04-15
期刊：
影响因子：
1.9
通讯作者：
Takai, O
中科院分区：
文献类型：
--
作者：
Ohta, R;Saito, N;Takai, O
应用原子力显微镜（AFM）和开尔文探针力显微镜（KPFM）对人血浆纤维蛋白原（HPF）进行了非接触式观察。HPF吸附在高度定向热解石墨（HOPG）基板上作为单分子，作为聚集束，和作为聚集纤维。地形和相位图像证实了暴露于空气后HPF的结构变化，而地形和KPFM图像证实了具有单个HPF分子宽度的纤维。此外，KPFM证实了HPF和HOPG之间的表面电位差，以及反映纤维中E和D畴的周期性电位降。(c)2006 Elsevier B.V.保留所有权利。
Human plasma fibrinogen (HPF) was observed by atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) conducted in non-contact mode. The HPF was adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate as single molecules, as aggregated bundles, and as aggregated fibers. Topographic and phase images confirmed structural changes in the HPF after exposure to air, while topographic and KPFM images confirmed fibers with the width of a single HPF molecule. Additionally, KPFM confirmed the surface potential difference between the HPF and the HOPG, and periodical potential drop reflecting the E and D domains in the fiber. (c) 2006 Elsevier B.V. All rights reserved.