Circulating levels of MCP-1 and IL-8 are elevated in human obese subjects and associated with obesity-related parameters
Circulating levels of MCP-1 and IL-8 are elevated in human obese subjects and associated with obesity-related parameters
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DOI：
10.1038/sj.ijo.0803259
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发表时间：
2006-09-01
影响因子：
4.9
通讯作者：
Yu, R.
中科院分区：
文献类型：
--
作者：
Kim, C-S;Park, H-S;Yu, R.
背景：趋化性细胞因子（chemotactic cytokines，简称chemokines）在白细胞转运中起重要作用.已经显示趋化因子的循环水平在炎症过程中增加，包括肥胖相关的病理（例如，G.动脉粥样硬化和糖尿病）。然而，目前对趋化因子与人类肥胖之间的关系知之甚少。在本研究中，我们调查了选定的趋化因子的循环水平（单核细胞趋化蛋白-1（MCP-1）、巨噬细胞炎性蛋白-1 α（MIP-1 α）、白细胞介素-1、白细胞介素-8（IL-8））以及趋化因子水平与肥胖相关参数之间的关联：体重指数（BMI）、腰围、空腹血糖和胰岛素水平、血脂谱和C反应蛋白（CRP）水平。共有100名受试者参加了本研究，其中50名肥胖（BMI>= 25 kg/m2），50名非肥胖（BMI <25 kg/m2）。采用双抗体夹心酶联免疫吸附法测定空腹血清中趋化因子和CRP水平。结果：肥胖组（BMI> 30kg/m2）血清MCP-1和IL-8水平明显高于非肥胖组（BMI <25kg/m2），差异有统计学意义（P <0.05）。CRP水平与BMI（P <0.001）、腰围（P <0.0001）呈正相关。MCP-1和IL-8水平与BMI（MCP-1，P <0.02; IL-8，P <0.01）和/或腰围（MCP-1，P <0.009; IL-8，P <0.03）呈正相关。MCP-1与CRP（P <0.007）、IL-6（P <0.0001）呈正相关，与HDL-胆固醇呈负相关（P <0.01）。结论：MCP-1、IL-8水平与肥胖相关的BMI、腰围、CRP、IL-6、HOMA、HDL-胆固醇等指标有关。这些发现表明，循环MCP-1和/或IL-8可能是一个潜在的候选人连接肥胖与肥胖相关的代谢并发症，如动脉粥样硬化和糖尿病。
Background: Chemotactic cytokines, referred to as chemokines, play an important role in leukocyte trafficking. The circulating levels of chemokines have been shown to increase in inflammatory processes including obesity-related pathologies (e. g. atherosclerosis and diabetes). However, little is currently known about the relationship between chemokines and human obesity. In the present study, we investigated the circulating levels of selected chemokines (monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1 alpha (MIP-1 alpha), leukotactin-1, interleukin-8 (IL-8)) and the association between the chemokine levels and obesity-related parameters: body mass index (BMI), waist circumference, fasting glucose and insulin levels, lipids profile, and the level of C-reactive protein (CRP).Methods: A total of 100 subjects, 50 obese (BMI >= 25 kg/m(2)) and 50 who were not obese (BMI < 25 kg/m(2)) participated in the present study. The levels of chemokines and CRP were measured in a fasting state serum by sandwich enzyme-linked immunosorbent assay. Total cholesterol, high-density lipoprotein (HDL)-cholesterol, triglyceride, glucose, and insulin levels were measured by enzymatic analysis and immunoassay.Results: The circulating levels of MCP-1 and IL-8 in the serum were significantly (P < 0.05) higher in obese subjects (BMI > 30 kg/ m 2) compared with those of nonobese controls (BMI < 25 kg/m(2)). The levels of CRP were positively correlated with BMI (P < 0.001) or waist circumference (P < 0.0001). The levels of MCP-1 and IL-8 were positively related to BMI (MCP-1, P < 0.02; IL-8, P < 0.01) and/ or waist circumference (MCP-1, P < 0.009; IL-8, P < 0.03). The levels of MCP-1 were positively related to the levels of CRP (P < 0.007) or interleukin-6 (IL-6) (P < 0.0001), and negatively related to the levels of HDL-cholesterol (P < 0.01). Homeostasis model assessment (HOMA) score was positively related to the levels of MCP-1 (P < 0.02) or IL-8 (P < 0.03) in obese subject.Discussion: Our data demonstrated that the circulating levels of MCP-1 and IL-8 are related to obesity-related parameters such as BMI, waist circumference, CRP, IL-6, HOMA and HDL-cholesterol. These findings suggest that the circulating MCP-1 and/ or IL-8 may be a potential candidate linking obesity with obesity-related metabolic complications such as atherosclerosis and diabetes.