STUDIES ON THE SUBSTRATE-SPECIFICITY AND INDUCIBILITY OF CYTOCHROME P-450MEG
STUDIES ON THE SUBSTRATE-SPECIFICITY AND INDUCIBILITY OF CYTOCHROME P-450MEG
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DOI：
10.1042/bj1960781
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发表时间：
1981-01-01
影响因子：
4.1
通讯作者：
RAFTER, JJ
中科院分区：
文献类型：
--
作者：
BERG, A;RAFTER, JJ
研究了巨型芽孢杆菌ATCC 13368中依赖于细胞色素P-450的类固醇15β-羟基酶系统：它在细胞中的出现与生物体的生长曲线有关，它被一些诱导剂(其中包括哺乳动物肝微粒体细胞色素P-450系统的一些经典诱导剂)诱导，以及它将非类固醇物质转化为含氧化合物的能力。在生长曲线的稳定期，该酶在细胞内达到最大浓度。在所有被测试的诱导剂中，没有一种显示出任何诱导细胞色素P-450meg的能力。在作为底物的被测试物质中，只有苯胺(对羟化)被微生物酶系统代谢。这种转化可能与血红素蛋白的一般加氧酶活性有关。BETA的底物特异性。巨噬细胞羟化酶体系明显狭窄。
The cytochrome P-450-dependent steroid 15.beta.-hydroxylase system in B. megaterium ATCC 13368 was investigated with regard to its appearance in the cell with respect to the growth curve of the organism, with regard to its inducibility by a number of agents (among them some of the classical inducers of the mammalian liver microsomal cytochrome P-450 system) and with regard to its capacity to convert non-steroidal substances into oxygenated compounds. The enzyme reached a maximum concentration in the cell during the stationary phase of the growth curve. Of all agents tested as inducers, none showed any capacity to induce cytochrome P-450meg. Of the substances tested as substrates only aniline (p-hydroxylation) was metabolized by the microbial enzyme system. This conversion might be related to the general oxygenase activity of hemoproteins. The substrate specificity of the .BETA.. megaterium hydroxylase system is apparently narrow.