Analysis of cytosine methylation status in potato by methylation-sensitive amplified polymorphisms under low-temperature stress
Analysis of cytosine methylation status in potato by methylation-sensitive amplified polymorphisms under low-temperature stress
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DOI：
10.1007/s12374-015-0316-1
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发表时间：
2015-11
影响因子：
2.9
通讯作者：
Cuihua Xin;Ruikun Hou;Fei Wu;Yibo Zhao;Huanhuan Xiao;Wantong Si;Md Emran Ali;L. Cai;Jiangbo Guo
中科院分区：
文献类型：
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作者：
Cuihua Xin;Ruikun Hou;Fei Wu;Yibo Zhao;Huanhuan Xiao;Wantong Si;Md Emran Ali;L. Cai;Jiangbo Guo
中国是世界上最大的马铃薯生产国。然而，低温胁迫会严重限制该国马铃薯作物的产量和品质。我们使用甲基化敏感的扩增多态性（MSAP）来分析这种压力如何可能改变马铃薯品种“拟南芥”的胞嘧啶甲基化状态。超过2400个基因座被确定和评估的DNA甲基化在冷冻处理和对照植物。胞嘧啶甲基化水平在处理48 h后从37.71%上升到56.51%，但在低温处理60 h后下降到42.14%。胁迫诱导的DNA甲基化带型变化主要是由于双链DNA上CG（5 '-CCGG-3'的内部胞嘧啶）的完全甲基化。我们对78个多态性DNA片段进行测序，并将它们与NCBI进行比对。然后通过半定量RT-PCR评估4个基因的表达模式。低温胁迫相关基因的表达模式受马铃薯甲基化或去甲基化状态的调控。
China is the leading producer of potatoes (Solanum tuberosum) in the world. However, low-temperature stress can severely restrict the yield and quality of potato crops in that country. We used methylation-sensitive amplified polymorphisms (MSAPs) to analyze how such stress might alter the status of cytosine methylation in potato cultivar ‘Atlantic’. Over 2400 loci were identified and assessed for DNA methylation in chilling-treated and control plants. The level of cytosine methylation increased from 37.71% to 56.51% after 48 h of treatment but then decreased to 42.14% after 60 h of chilling. Stress-induced changes in the banding patterns of DNA methylation primarily resulted from full methylation of CG (internal cytosine of 5’-CCGG-3’) on double DNA strands. We sequenced 78 polymorphic DNA fragments and blasted them against NCBI. Expression patterns of 4 genes were then assessed via semi-quantitative RT-PCR. The expression patterns of genes related to chilling stress appeared to be regulated by the methylation or demethylation status in potato.