A novel procedure for generating both nitric oxide and superoxide in situ from chemical sources at any chosen mole ratio. First application: Tyrosine oxidation and a comparison with preformed peroxynitrite
A novel procedure for generating both nitric oxide and superoxide in situ from chemical sources at any chosen mole ratio. First application: Tyrosine oxidation and a comparison with preformed peroxynitrite
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DOI：
10.1021/tx0001272
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发表时间：
2000-12-01
影响因子：
4.1
通讯作者：
Ingold, KU
中科院分区：
文献类型：
--
作者：
Hodges, GR;Marwaha, J;Ingold, KU
生成的第一种方法。NO和O-2（.-）在任何已知的恒定比率下，已经开发了。精胺NONOate和二（4-羧基苄基）次亚硝酸盐在37 ℃和pH 7.5下以一级动力学和完全相等的速率常数（半衰期为80 min）衰减，得到200和40 mol %。NO和O-2（.-），分别酪氨酸氧化为二酪氨酸和3-硝基酪氨酸（在这些实验中使用的条件下的主要和次要产物）已被研究（主要在CO2的存在下），使用各种不同的比率的形成。NO和O-2（.-）。的. NO/O-2（.-）= 1.0的产品曲线与等量的衍生产品非常相似。NO和O-2（.-）生成于A。NO/O-2（.-）与SIN-1的比率为1.0，但与衍生自预形成的过氧亚硝酸盐的那些非常不同。所有实验结果都可用自由基化学的观点加以解释。产品简介在所有的。NO/O（2）（.-）只要包括导致二酪氨酸消耗的一组重要反应，就可以令人满意地模拟这些比率。
The first method for generating . NO and O-2(.-) at any known, constant ratio has been developed. Spermine NONOate and di(4-carboxybenzyl)hyponitrite decay with first-order kinetics and exactly equal rate constants (half-lives of 80 min) at 37 degreesC and pH 7.5 to give 200 and 40 mol % . NO and O-2(.-), respectively. Tyrosine oxidation to dityrosine and 3-nitrotyrosine (the major and minor products under the conditions used in these experiments) has been studied (mainly in the presence of CO2) using various different ratios of the rates of formation of . NO and O-2(.-). The . NO/O-2(.-) = 1.0 product profiles are very similar to those of the products derived from equal amounts of . NO and O-2(.-) generated at a . NO/O-2(.-) ratio of 1.0 from SIN-1 but are very different from those derived from preformed peroxynitrite. All the experimental results can be explained in terms' of free radical chemistry. The product profiles at all the . NO/O(2)(.-)ratios could be satisfactorily simulated provided an important group of reactions which lead to the consumption of dityrosine was included.