DETECTION OF EPSTEIN-BARR-VIRUS DNA BY INSITU HYBRIDIZATION AND POLYMERASE CHAIN-REACTION IN SALIVARY-GLAND BIOPSY SPECIMENS FROM PATIENTS WITH SJOGREN SYNDROME
DETECTION OF EPSTEIN-BARR-VIRUS DNA BY INSITU HYBRIDIZATION AND POLYMERASE CHAIN-REACTION IN SALIVARY-GLAND BIOPSY SPECIMENS FROM PATIENTS WITH SJOGREN SYNDROME
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DOI：
10.1016/0002-9343(91)80007-9
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发表时间：
1991-03-01
影响因子：
5.9
通讯作者：
MORINET, F
中科院分区：
文献类型：
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作者：
MARIETTE, X;GOZLAN, J;MORINET, F
目的：确定 Epstein-Barr 病毒 (EBV) 是否可能参与干燥综合征 (SS) 的发病机制。 患者和方法：使用 EBV DNA BamH1-W 片段对 14 名 SS 患者（8 名原发性 SS 和 6 名继发性 SS）和 39 名对照受试者的唇唾液腺活检标本进行原位杂交。此外，对 12 名 SS 患者（7 名原发性 SS 和 5 名继发性 SS）和 24 名对照受试者的唇唾液腺活检标本进行聚合酶链反应检测 EBV DNA。 结果：原位杂交在 8 名原发性 SS 患者中的 4 名（50%）、6 名继发性 SS 患者中的 0 名以及 39 名中的 3 名患者的唇唾液腺活检标本的上皮细胞中检测到 EBV DNA。 (8%) 对照受试者。原发性 SS 患者与对照受试者之间的差异具有统计学意义 (p < 0.02)。聚合酶链反应在 7 名原发性 SS 患者中的 6 名 (86%)、5 名继发性 SS 患者中的 3 名 (60%) 以及 24 名对照受试者中的 7 名 (29%) 中检测到了 EBV DNA。原发性 SS 患者和对照受试者之间的差异具有统计学意义 (p < 0.01)。 结论：两种新开发的技术均表明，与对照受试者相比，原发性 SS 患者中 EBV DNA 的存在显着增加。所有原位杂交阳性SS患者中，唇唾液腺上皮细胞都是EBV感染的目标。我们的结果表明该病毒可能在 SS 的发病机制中发挥作用。我们还不能确定 EBV 是否直接导致腺体破坏，或者它的存在是否是腺体损伤后的继发事件。
PURPOSE: To determine whether Epstein-Barr virus (EBV) could be involved in the pathogenesis of Sjogren's syndrome (SS).PATIENTS AND METHODS: In situ hybridization using the BamH1-W fragment of EBV DNA was performed using labial salivary gland biopsy specimens from 14 patients with SS (eight with primary SS and six with secondary SS) and 39 control subjects. Furthermore, labial salivary gland biopsy specimens from 12 patients with SS (seven with primary SS and five with secondary SS) and 24 control subjects were submitted to the polymerase chain reaction to detect EBV DNA.RESULTS: In situ hybridization detected EBV DNA in epithelial cells of labial salivary gland biopsy specimens from four of eight (50%) patients with primary SS, zero of six patients with secondary SS, and three of 39 (8%) control subjects. The difference between patients with primary SS and control subjects was statistically significant (p < 0.02). The polymerase chain reaction detected EBV DNA in six of seven (86%) patients with primary SS, three of five (60%) patients with secondary SS, and seven of 24 (29%) control subjects. The difference between patients with primary SS and control subjects was statistically significant (p < 0.01).CONCLUSION: Both newly developed techniques showed that the presence of EBV DNA was significantly increased in patients with primary SS in comparison with control subjects. In all the positive SS patients who underwent in situ hybridization, epithelial cells of the labial salivary gland were the target of EBV infection. Our results suggest that this virus may play a role in the pathogenesis of SS. We cannot yet determine whether EBV is directly responsible for the destruction of the gland, or if its presence is a secondary event following gland injury.