Enhancement of neutralizing activity of influenza virus-specific antibodies by serum components
Enhancement of neutralizing activity of influenza virus-specific antibodies by serum components
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DOI：
10.1016/j.virol.2006.05.008
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发表时间：
2006-09-01
期刊：
影响因子：
3.7
通讯作者：
Gerhard, Walter
中科院分区：
文献类型：
--
作者：
Mozdzanowska, Krystyna;Feng, JingQi;Gerhard, Walter
研究了血清组分对流感病毒A/PR/8/34血凝素（HA）特异性单克隆抗体（MAb）体外中和活性的影响。增强的程度取决于单克隆抗体的精细特异性和重链同种型和血清类型。在缺乏血清免疫球蛋白的免疫缺陷小鼠的血清中观察到最大的增强（> 100倍）。至少有两种血清成分参与：C1 q和耐热因子。C1 q对于增强是强制性的，并且不需要补体系统的其他组分。C1 q似乎通过改善单克隆抗体介导的病毒附着到宿主细胞的抑制作用而起作用，并且对于自身抑制病毒附着较差的单克隆抗体最有效。耐热因子增强VN活性只有在C1 q的存在下，并出现通过增强VN活性在后附着阶段的操作。(c)2006年爱思唯尔公司All rights reserved.
The role of serum components in enhancing virus neutralizing (VN) activity of influenza virus A/PR/8/34 hemagglutinin (HA)-specific MAbs in vitro was investigated. The degree of enhancement depended on the MAb's fine specificity and heavy chain isotype and on type of serum. Greatest enhancement (> 100-fold) was seen with sera from immunodeficient mice that lacked serum immunoglobulin. At least two serum components were involved: C1q and a heat-resistant factor. C1q was mandatory for enhancement, and other components of the complement system were not required. C1q appeared to operate by improving MAb-mediated inhibition of virus attachment to host cells and was most effective with MAbs that inhibited virus attachment poorly on their own. The heat-resistant factor enhanced VN activity only in the presence of C1q and appeared to operate by enhancing VN activity at a post-attachment stage. (c) 2006 Elsevier Inc. All rights reserved.