Mossy fiber sprouting in the fascia dentata after unilateral entorhinal lesions: quantitative analysis using computer-assisted image processing.
Mossy fiber sprouting in the fascia dentata after unilateral entorhinal lesions: quantitative analysis using computer-assisted image processing.
复制标题
单侧内嗅病变后齿状筋膜中苔藓纤维发芽：使用计算机辅助图像处理进行定量分析。
DOI：
10.1016/0306-4522(84)90237-9
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Dewey,SL
中科院分区：
文献类型：
--
作者：
West,JR;Dewey,SL
轴突发芽通常发生在部分失神经支配的大脑区域。本研究表明，定量，发芽的区域外的deafferentation的证据。一个修改的Timm硫化物银组织化学方法被用来监测增加苔藓纤维终末领域的成年大鼠后严重deafferentation的外部四分之三的层moleculare由单侧内嗅病变的筋膜。计算机辅助图像处理技术被用来量化苔藓纤维发芽。在颗粒层中，以及在较小程度上在分子层的深层（颗粒上）部分（与传入神经阻滞区分离的区域）中，与未手术侧（和未手术动物）相比，病变侧苔藓纤维染色面积增加了3倍。由于苔藓纤维发芽明显发生在该地区的退化产生的突触稀释的情况下，它可能代表了一个例子，损伤后的增长，从根本上不同的条件下，通常认为是诱导发芽。
Axon sprouting typically occurs in a brain region that has been partially denervated. The present study demonstrates, quantitatively, evidence for sprouting outside the region of deafferentation. A modification of the Timm sulfide silver histochemical method was used to monitor an increase in the mossy fiber terminal field in the fascia dentata of adult rats following severe deafferentation of the outer three-fourths of stratum moleculare by unilateral entorhinal lesions. Computer-assisted image processing techniques were used to quantify mossy fiber sprouting. In stratum granulosum, and to a lesser extent in the deep (supragranular) portion of stratum moleculare (areas separated from the zone of deafferentation), there was a three-fold increase in the area of mossy fiber staining on the side of the lesion compared to the non-operated side (and unoperated animals). Much of the increased staining was located near the tip of the infrapyramidal (ventral) blade of the fascia dentata.Since mossy fiber sprouting apparently occurs in the absence of degeneration-produced synaptic dilution in that region, it may represent an example of post-lesion growth initiated by conditions fundamentally different from those normally believed to induce sprouting.