Agarose Slurry as a Support Medium for Bioprinting and Culturing Freestanding Cell-Laden Hydrogel Constructs
Agarose Slurry as a Support Medium for Bioprinting and Culturing Freestanding Cell-Laden Hydrogel Constructs
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DOI：
10.1089/3dp.2018.0175
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发表时间：
2019-06-01
影响因子：
3.1
通讯作者：
Sarvazyan, Narine
中科院分区：
文献类型：
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作者：
Mirdamadi, Eman;Muselimyan, Narine;Sarvazyan, Narine
我们提出了一种改进的嵌入式生物打印方法，它允许在细胞培养条件下长时间保持独立的三维（3D）打印结构。这种方法被称为CLASS（构建物放置在琼脂糖浆液悬浮液中），使用细胞负载海藻酸盐和凝胶甲基丙烯酸酯（GelMa）为基础的生物墨水进行了测试。直接比较明胶和琼脂糖水凝胶浆液支持的3D打印结构，揭示了几个优势，包括浆液在不同打印温度和混合时间下的稳定性，浆液均匀性的增加，以及CLASS在细胞培养中延长支持独立结构的能力。我们得出的结论是，CLASS是一种直接且经济有效的打印和支持独立细胞负载生物材料的方法。
We present a modified method of embedded bioprinting, which allows maintaining freestanding three-dimensional (3D) printed structures in cell culture conditions for extended periods of time. This method, termed CLASS (constructs laid in agarose slurry suspension), was tested using cell-laden alginate and gelatin methacrylate (GelMa)-based bioinks. A direct comparison of 3D printed constructs, supported by gelatin and agarose hydrogel slurries, revealed several advantages, including slurry stability across different print temperatures and blending times, increased slurry homogeneity, and the ability of CLASS to support freestanding constructs for an extended time in cell culture. We conclude that CLASS is a straightforward and cost-efficient way to print and support freestanding cell-laden biomaterials.