DNA content determination of micronucleated polychromatic erythrocytes induced by clastogens and spindle poisons in mouse bone marrow and peripheral blood.
DNA content determination of micronucleated polychromatic erythrocytes induced by clastogens and spindle poisons in mouse bone marrow and peripheral blood.
复制标题
小鼠骨髓和外周血中断裂剂和纺锤体毒物诱导的微核嗜多染红细胞DNA含量测定。
DOI：
10.1093/mutage/8.3.249
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发表时间：
1993
期刊：
影响因子：
2.7
通讯作者：
H. Amnéus
中科院分区：
文献类型：
--
作者：
J. Grawé;G. Zetterberg;H. Amnéus
用流式细胞仪检测了小鼠骨髓和外周血嗜多染红细胞（PCE）中微核（MN）的频率和DNA分布。使用荧光RNA染色剂噻唑橙子将PCE与正染性红细胞（NCE）区分开，而使用DNA染色剂Hoechst 33342检测红细胞中的MN。处理包括X线照射（1戈伊）、环磷酰胺（CPA; 30 mg/kg）、硫酸长春新碱（VCR; 0.08 mg/kg）和胆固醇（COL; 1 mg/kg）。所有处理均显示BM处理后30 h和PB处理后50 h的微核PCE频率增加。染色体断裂剂（X射线和CPA）和纺锤体毒物（VCR和COL）可根据诱导MN的荧光特性以及小MN（占二倍体DNA含量的0.5-2%）和大MN（占二倍体DNA含量的2-10%）的相对频率进行分组。在PB中，大MN的相对频率低于BM，表明它们在进入外周循环之前被部分消除。
The frequencies and DNA distributions of micronuclei (MN) in polychromatic erthrocytes (PCEs) from bone marrow (BM) and peripheral blood (PB) of mice after four different treatments were determined by flow cytometry. PCEs were differentiated from normochromatic erythrocytes (NCEs) using the fluorescent RNA stain Thiazole orange, while MN in erythrocytes were detected with the DNA stain Hoechst 33342. The treatments were X-irradiation (1 Gy), cyclophosphamide (CPA; 30 mg/kg), vincristine sulphate (VCR; 0.08 mg/kg), and cholchicine (COL; 1 mg/kg). All treatments showed increased frequencies of micronucleated PCEs at 30 h after treatment in BM and at 50 h in PB. The clastogens (X-irradiation and CPA) and the spindle poisons (VCR and COL) could be grouped according to the fluorescent characteristics of the induced MN as well as the relative frequency of small (0.5-2% of the diploid DNA content) and large (2-10%) MN. In PB the relative frequency of large MN was lower than in BM, indicating that they were partly eliminated before entrance into the peripheral circulation.