AUTOMATED FLUOROMETRIC AMINO-ACID ANALYSIS - DETERMINATION OF PROLINE AND HYDROXYPROLINE
AUTOMATED FLUOROMETRIC AMINO-ACID ANALYSIS - DETERMINATION OF PROLINE AND HYDROXYPROLINE
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DOI：
10.1016/0003-2697(79)90366-x
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发表时间：
1979-01-01
影响因子：
2.9
通讯作者：
MELLET, M
中科院分区：
文献类型：
--
作者：
BOHLEN, P;MELLET, M
本文建立了一种自动测定脯氨酸和羟脯氨酸的荧光光度法。该测定是基于柱后反应的亚氨基酸与碱性次氯酸钠，这产生的氧化产物适合检测与荧光胺试剂。该方法简单，适用于邻苯二甲醛检测的高灵敏度氨基酸分析仪。可准确测定低至10 pmol的脯氨酸和20 pmol的羟脯氨酸。因此，现在可以使用邻苯二甲醛在高灵敏度氨基酸分析仪上测定天然亚氨基和氨基酸的全部序列。
A fluorometric method for the automated determination of the imino acids proline and hydroxyproline has been developed. The assay is based on the postcolumn reaction of the imino acids with alkaline sodium hypochlorite, which yields oxidation products amenable to detection with fluorogenic amine reagents. The method is simple and can be adapted readily to high-sensitivity amino acid analyzers which use o-phthalaldehyde for detection. As little as 10 pmol proline and 20 pmol hydroxyproline can be determined accurately. Thus the full array of natural imino and amino acids can now be determined on a high-sensitivity amino acid analyzer using o-phthalaldehyde.