Characteristics of the intestinal flora of specific pathogen free chickens with age
Characteristics of the intestinal flora of specific pathogen free chickens with age
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无特定病原体鸡肠道菌群随年龄变化的特征
DOI：
10.1016/j.micpath.2019.05.014
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发表时间：
2019-07-01
影响因子：
3.8
通讯作者：
Feng Xin
中科院分区：
文献类型：
--
作者：
Yu Xi;Niu Shuling;Feng Xin
无特定病原体(SPF)实验动物是生物医学、畜牧业和兽医研究与生产领域公认的标准实验动物。肠道菌群在营养吸收、改善健康和保护宿主免受病原体侵袭方面起着至关重要的作用。因此，我们对SPF雏鸡肠道菌群的变化和维持进行了探讨，以期更好地了解SPF鸡肠道菌群的组成，为SPF实验动物肠道菌群的研究提供参考。分别在14日龄、28日龄和42日龄各随机抽取5只雏鸡，取出盲肠进行DNA提取。利用Illumina MiSeq平台对16S rRNA基因的V3-V4区进行微生物组分析。在雏鸡肠道微生物组的发育过程中，我们观察到了多样性的主要变化，特别是在第14天到第28天之间。细菌类群以菌丝体、变形杆菌和类杆菌为主，且菌丝体数量随年龄的增加而显著增加。相对丰度最高的属是乳杆菌属，其次是杆菌属。此外，瘤胃球菌科、瘤胃球菌和布鲁氏菌的丰度随着年龄的增加而增加，而乳杆菌、肠杆菌科和振荡杆菌的丰度随着年龄的增加而减少。有趣的是，随着时间的推移，粪便杆菌的丰度先增加后减少。SPF鸡不同日龄肠道菌群的特点为育雏期早期肠道菌群的调控奠定了基础，也为新生鸡感染和禽病的防控提供了理论依据。
Specific pathogen-free (SPF) experimental animals are recognized as standard laboratory animals in the fields of biomedical, animal husbandry and veterinary research and production. Intestinal flora plays a critical role in nutrient absorption, improving health and protecting the host from pathogens. We therefore explored the variation and maintenance of intestinal flora in SPF chicks in order to better understand the composition of intestinal microflorain SPF chickens, and provide reference for the study of intestinal flora of SPF experimental animals. Five chicks were randomly selected at each of 14, 28, and 42 days, and ceca were removed for DNA extraction. The Illumina Miseq platform was used for microbiome analysis of the V3-V4 region of the 16S rRNA gene. During the course of chick gut microbiome development, we observed major changes in diversity, especially between day 14 and day 28. Firmicutes, Proteobacteria, and Bacteroidetes were the main bacterial taxa, and Firmicutes increased significantly with age. The genus with the highest relative abundance was Lactobacillus, followed by Faecalibacterium. In addition, while abundance of Ruminococcaceae spp., Ruminococcus, and Blautia increased with age, Lactobacillus, Enterobacteriaceae spp., and Oscillospira decreased with age. Interestingly, the abundance of Faecalibacterium first increased and then decreased over time. The characteristics of SPF chicken gut flora at different ages establish a basis for the regulation of intestinal flora in the early stage of brooding, and also provide a theoretical foundation for controlling and preventing infections and poultry diseases in newborn chickens.