Diphenylarsinic acid produces behavioral effects in mice relevant to symptoms observed in citizens who ingested polluted well water
Diphenylarsinic acid produces behavioral effects in mice relevant to symptoms observed in citizens who ingested polluted well water
复制标题
DOI：
10.1016/j.ntt.2011.08.007
复制
发表时间：
2012-01-01
影响因子：
2.9
通讯作者：
Morita, Masatoshi
中科院分区：
文献类型：
--
作者：
Umezu, Toyoshi;Nakamiya, Kunichika;Morita, Masatoshi
神津市某地区的市民。日本茨木市出现了不寻常的健康问题，在该地区发现了二苯胂酸（DPAA）对井水的污染。我们研究了DPAA对小鼠各种行为的影响。通过自由饮用水给小鼠施用DPAA 27周（亚慢性暴露）或57周（慢性暴露），并在暴露期间检查行为。30-100 ppm的DPAA增加小鼠的行走活动和穿梭型离散条件回避反应的反应率。DPAA在100 ppm时降低了固定棒上的配位能力。7.5-15 ppm的DPAA也降低了旋转杆上的协调性，尽管这些剂量的DPAA不影响固定杆上的协调性。慢性暴露于7.5-15 ppm的DPAA在高架十字迷宫试验中产生抗焦虑样作用，而亚慢性暴露于100 ppm的DPAA产生焦虑样作用。无论是亚慢性或慢性暴露于7.5-100 ppm的DPAA影响学习能力和/或记忆，使用被动回避反应进行评估。暴露于15-30 ppm的DPAA 52周不会改变小鼠大脑和小脑的重量或小脑中神经元标记蛋白TUJ-1或星形胶质细胞标记蛋白胶质细胞酸性蛋白的量。在小鼠中观察到的行为影响似乎与在Kamisu市患者中观察到的症状有关。(C)2011 Elsevier Inc. All rights reserved.
Citizens in an area of Kamisu City. Ibaraki, Japan had exhibited unusual health problems, and pollution of well water by diphenylarsinic acid (DPAA) was found in the area. We examined the effects of DPAA on various behaviors in mice. DPAA was administered to mice through free intake of drinking water for 27 weeks (subchronic exposure) or 57 weeks (chronic exposure), and behavior was examined during exposure. DPAA at 30-100 ppm increased ambulatory activity and the response rate of the shuttle type discrete conditioned avoidance response of mice. DPAA reduced coordination ability on the fixed rod at 100 ppm. DPAA at 7.5-15 ppm also reduced coordination on the rotating rod, although these doses of DPAA did not affect coordination on the fixed rod. Chronic exposure to 7.5-15 ppm of DPAA produced anti-anxiety-like effects in the elevated plus maze test, whereas subchronic exposure to 100 ppm of DPAA produced anxiogenic-like effects. Neither subchronic nor chronic exposure to 7.5-100 ppm of DPAA affected learning ability and/or memory, as evaluated using the passive avoidance response. Exposure to 15-30 ppm of DPAA for 52 weeks did not alter weights of the cerebrum and cerebellum or amounts of neuron marker protein TUJ-1 or astrocyte marker protein glial fibrillary acidic protein in the cerebellum of mice. Behavioral effects observed in mice seem relevant to symptoms observed in patients from Kamisu City. (C) 2011 Elsevier Inc. All rights reserved.