Coupled oscillators control morning and evening locomotor behaviour of Drosophila
Coupled oscillators control morning and evening locomotor behaviour of Drosophila
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DOI：
10.1038/nature02926
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发表时间：
2004-10-14
期刊：
影响因子：
64.8
通讯作者：
Rosbash, M
中科院分区：
文献类型：
--
作者：
Stoleru, D;Peng, Y;Rosbash, M
生理和行为的日常节奏是由内源性生物钟精确计时的（1,2）。这包括早晚分开活动，这是黑腹果蝇和许多其他类群（包括哺乳动物）的特征（3-5）。尽管长期以来一直有人提出多个振荡器来协调这种复杂的行为程序(6)，但它们的性质和相互作用仍然难以捉摸。通过细胞特异性消融，我们发现果蝇早晚活动的时间来自两组不同的昼夜节律神经元：早晨活动来自表达神经肽PDF的腹侧神经元，晚上活动来自另一组细胞，包括背侧神经元。尽管这两个振荡器可以自主工作，但针对生物钟突变体的细胞特异性救援实验表明，它们在功能上是耦合的。
Daily rhythms of physiology and behaviour are precisely timed by an endogenous circadian clock(1,2). These include separate bouts of morning and evening activity, characteristic of Drosophila melanogaster and many other taxa, including mammals(3-5). Whereas multiple oscillators have long been proposed to orchestrate such complex behavioural programmes(6), their nature and interplay have remained elusive. By using cell-specific ablation, we show that the timing of morning and evening activity in Drosophila derives from two distinct groups of circadian neurons: morning activity from the ventral lateral neurons that express the neuropeptide PDF, and evening activity from another group of cells, including the dorsal lateral neurons. Although the two oscillators can function autonomously, cell-specific rescue experiments with circadian clock mutants indicate that they are functionally coupled.