Rapid additive-free bacteria lysis using traveling surface acoustic waves in microfluidic channels
Rapid additive-free bacteria lysis using traveling surface acoustic waves in microfluidic channels
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DOI：
10.1039/c9lc00656g
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发表时间：
2019-12-21
期刊：
影响因子：
6.1
通讯作者：
Weitz, David A.
中科院分区：
文献类型：
--
作者：
Lu, Haiwei;Mutafopulos, Kirk;Weitz, David A.
我们报告了一种无添加剂的方法，裂解细菌和提取核酸和蛋白质使用的表面声波（TSAW）耦合到一个微流控装置。我们描述了TSAW对E.通过测量暴露在声波下的细胞的生存能力，发现大约90%的细胞死亡。此外，我们测量了从细胞中释放的蛋白质和核酸，并表明我们回收了约20%的总物质。裂解方法应适用于所有类型的细菌。这些结果证明了使用TSAW以不依赖于细菌类型的方式裂解细菌的可行性。
We report an additive-free method to lyse bacteria and extract nucleic acids and protein using a traveling surface acoustic wave (TSAW) coupled to a microfluidic device. We characterize the effects of the TSAW on E. coli by measuring the viability of cells exposed to the sound waves and find that about 90% are dead. In addition, we measure the protein and nucleic acids released from the cells and show that we recover about 20% of the total material. The lysis method should work for all types of bacteria. These results demonstrate the feasibility of using TSAW to lyse bacteria in a manner that is independent of the type of bacteria.