Extinction of differentiated glial functions in somatic ce-l hybrids is incomplete.
Extinction of differentiated glial functions in somatic ce-l hybrids is incomplete.
复制标题
体细胞杂种中分化的神经胶质功能的消失是不完全的。
DOI：
10.1016/0014-4827(78)90150-7
复制
发表时间：
1978
影响因子：
3.7
通讯作者：
D. Post
中科院分区：
文献类型：
--
作者：
F. Mcmorris;D. Post
将克隆C6的大鼠神经胶质瘤细胞与克隆A9的小鼠L细胞或新鲜分离的小鼠巨噬细胞进行体外杂交，并测定杂交体的神经胶质细胞功能。 C6细胞表达高水平的2',3'-环核苷酸3'-磷酸水解酶（CNP；EC 3.1.4.37）、β-羟基丁酸脱氢酶（HBDH；EC 1.1.1.30）、3-磷酸甘油脱氢酶（GPDH；EC 1.1.1.8）以及氢化可的松（HC）诱导的GPDH。 A9细胞和巨噬细胞这些功能的活性非常低。 C6和A9之间或C6和巨噬细胞之间的杂交体大大降低了这些功能的活性，但杂交体表达的活性显着高于非神经胶质亲本。这种不完全灭绝并不是由于两个神经胶质瘤细胞与一个 L 细胞或巨噬细胞融合所致。与非神经胶质亲本相比，杂交体中 GPDH 活性的差异是由于神经胶质亲本的 GPDH 不完全关闭，而不是由于非神经胶质基因组 GPDH 产量的增加。
Rat glioma cells of clone C6were hybridized in vitro with mouse L cells of clone A9 or with freshly isolated mouse macrophages, and the hybrids were assayed for glial cell functions. C6cells expressed high levels of 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP; EC 3.1.4.37), β-hydroxybutyrate dehydrogenase (HBDH; EC 1.1.1.30), glycerol-3-phosphate dehydrogenase (GPDH; EC 1.1.1.8), and inducibility of GPDH by hydrocortisone (HC). A9 cells and macrophages had very low activities of these functions. Hybrids between C6and A9 or between C6and macrophages had greatly reduced activities of these functions, but the hybrids expressed significantly higher activities than the non-glial parent. This incomplete extinction was not due to fusion of two glioma cells with one L cell or macrophage. The difference in GPDH activity in the hybrids as compared with the non-glial parent was due to incomplete shut-off of GPDH of the glial parent, and not to an increase in GPDH production by the non-glial genome.