Antioxidant actions of nitroxyl (HNO)
Antioxidant actions of nitroxyl (HNO)
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DOI：
10.1016/j.freeradbiomed.2006.11.015
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发表时间：
2007-02-15
影响因子：
7.4
通讯作者：
Fukuto, Jon M.
中科院分区：
文献类型：
--
作者：
Lopez, Brenda E.;Shinyashiki, Masaru;Fukuto, Jon M.
氮氧化物是内源性产生的信号/效应分子，具有引起和改善氧化应激的潜力。氮氧化物是否作为氧化剂或抗氧化剂取决于许多因素，包括细胞环境，浓度和其他活性物质的存在。迄今为止，氮氧化物硝酰基（HNO）仅被报道具有促氧化剂性质。然而，它的一些化学性质可以预测它也可以作为抗氧化剂。在这项研究中，HNO可能的抗氧化作用进行了研究，使用酵母酿酒酵母模型系统。HNO对膜脂过氧化的影响进行了研究，并确定HNO在该系统中仅作为抗氧化剂。在谷胱甘肽的存在下，一种含巯基的肽，清除HNO，抗氧化作用降低。此外，HNO的抗氧化性能是不是由于HNO的NO的转换。这些结果也证实了在体外试验的氧化应激。因此，HNO具有通过其抗氧化作用来保持脂质膜完整性的潜力。(c)2006年爱思唯尔公司All rights reserved.
Nitrogen oxides are endogenously produced signaling/effector molecules that have the potential to both cause and ameliorate oxidative stress. Whether nitrogen oxides behave as oxidants or antioxidants is dependent on many factors including the cellular environment, the concentration, and the presence of other reactive species. To date, the nitrogen oxide nitroxyl (HNO) has only been reported to possess prooxidant properties. However, some of its chemical properties would predict that it could also serve as an antioxidant. In this study, the possible antioxidant actions of HNO were examined using the yeast Saccharomyces cerevisiae model system. The effect of HNO on membrane lipid peroxidation was examined and HNO was determined to act solely as an antioxidant in this system. In the presence of glutathione, a thiol-containing peptide that scavenges HNO, the antioxidant action was decreased. In addition, the antioxidant properties of HNO were not due to the conversion of HNO to NO. These results were also confirmed with in vitro assays of oxidative stress. Thus, HNO has the potential to preserve lipid membrane integrity by its antioxidant actions. (c) 2006 Elsevier Inc. All rights reserved.