One-Stage Factor VIII Assays
One-Stage Factor VIII Assays
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DOI：
10.1007/978-1-4939-7196-1_20
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Rodgers, Susan
中科院分区：
文献类型：
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作者：
Duncan, Elizabeth;Rodgers, Susan
使用一步法的凝血因子测定广泛用于测量因子水平，以检测缺陷或监测替代疗法。在本章中，我们重点介绍了一种通过一期试验（FVIII：C-1）测量因子VIII凝血活性（FVIII：C）的方法，提供了额外信息，以使该方法也适用于因子IX（FIX）、因子XI（FXI）和因子XII（FXII）。从实验室检测的角度来看，这些因素是“内在”凝血途径的组成部分，并且都是在基于活化部分凝血活酶时间（APTT）校正的测试系统中测量的。通过测量未知样本相对于已知校准品的效应校正因子缺陷血浆（目标因子缺陷）延长APTT的能力来评估因子活性。这些检测用于诊断因子水平降低的许多原因，包括导致APTT延长的原因。实验室工作人员了解方法变化、限制和结果解释的影响是很重要的，这些方面也进行了讨论。
Coagulation factor assays using one-stage methodology are widely used to measure factor levels for the purpose of detecting a deficiency, or to monitor replacement therapy. In this chapter, we focus on a method to measure Factor VIII coagulant activity (FVIII:C) by the one-stage assay (FVIII:C-1), with extra information provided to also allow this method to apply to Factor IX (FIX), Factor XI (FXI), and Factor XII (FXII). From the perspective of laboratory testing, these factors are components of the "intrinsic" coagulation pathway and are all measured in test systems based on the correction of the Activated Partial Thromboplastin Time (APTT). Factor activity is assessed by measuring the ability of an unknown sample to correct the prolonged APTT of factor-deficient plasma (deficient in the factor of interest), relative to the effect of a known calibrator. These assays are used for the diagnosis of the many causes of reduced factor levels, including those causing a prolonged APTT. It is important for laboratory staff to understand the impact of method variations, limitations, and result interpretation and these aspects are also discussed.