Stress fibers stabilize the position of intranuclear DNA through mechanical connection with the nucleus in vascular smooth muscle cells
Stress fibers stabilize the position of intranuclear DNA through mechanical connection with the nucleus in vascular smooth muscle cells
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DOI：
10.1016/j.febslet.2011.11.006
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发表时间：
2011-12-15
期刊：
影响因子：
3.5
通讯作者：
Matsumoto, Takeo
中科院分区：
文献类型：
--
作者：
Nagayama, Kazuaki;Yahiro, Yuki;Matsumoto, Takeo
应用激光纳米切割技术，对血管平滑肌细胞核顶面的肌动蛋白应力纤维（Actin stress fibers，SFs）进行了切割，以释放其预张力，并研究了SFs、细胞核和核内DNA的动力学行为。解剖后，细胞核顶面的SF缩短。细胞核向丝泡收缩方向移动，并出现明显的局部变形，表明丝泡与细胞核表面紧密相连。细胞核内DNA的分布发生了明显的变化，位于细胞周围和附近的DNA消失。这些结果表明，SFs通过与细胞核的机械连接来稳定核内染色质的位置。SFs的张力可能通过机械方式传递到细胞核，引起核内染色质的构象变化。(C)2011年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Actin stress fibers (SFs) running across the top surface of the nucleus in vascular smooth muscle cells were dissected using laser nano-dissection technique to release its pretension, and the dynamic behavior of SFs, nucleus, and intranuclear DNA were investigated. SFs shortened across the top surface of the nuclei after their dissection. The nuclei moved in the direction of SF retraction, and showed marked local deformation, indicating that SFs firmly connected to the nuclear surface. Intranuclear DNA located near and around the dissected SFs disappeared and their distribution changed markedly. These findings suggest that SFs stabilize the position of intranuclear chromatin through mechanical connection with the nucleus. The tension of SFs may be transmitted mechanically to the nucleus inducing conformational changes of intranuclear chromatin. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.