Analysis of phosphate position in hexose monosaccharides using ion-molecule reactions and SORI-CID on an FT-ICR mass spectrometer.
Analysis of phosphate position in hexose monosaccharides using ion-molecule reactions and SORI-CID on an FT-ICR mass spectrometer.
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在 FT-ICR 质谱仪上使用离子分子反应和 SORI-CID 分析己糖单糖中的磷酸位置。
DOI：
10.1021/ac020054m
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发表时间：
2002
影响因子：
7.4
通讯作者：
Leary,JulieA
中科院分区：
文献类型：
--
作者：
Leavell,MichaelD;Kruppa,GaryH;Leary,JulieA
通过离子−分子反应和SORI-CID，在负离子模式下确定了己糖磷酸单糖中的磷酸位置。使用硼酸三甲酯作为试剂气体，发现它很容易与磷酸化的己糖单糖反应。试剂气与磷酸己糖反应后，用Sori-CID对前驱体进行离子活化，得到不同的MS/MS图谱。对两种异构体产生不同的诊断离子，从而能够区分和确定磷酸部分的连接位置。
Through the use of ion−molecule reactions and SORI-CID, the phosphate position in hexose phosphate monosaccharides has been determined in the negative ion mode. Trimethyl borate was used as a reagent gas and was found to react readily with the phosphorylated hexose monosaccharides. After reaction of the reagent gas with the hexose phosphate, ion activation of the precursor by SORI-CID yielded different MS/MS spectra. Different diagnostic ions were generated for the two isomers, thus enabling differentiation and linkage position determination of the phosphate moiety.