Alterations in protein expression caused by the hha mutation in Escherichia coli:: Influence of growth medium osmolarity
Alterations in protein expression caused by the hha mutation in Escherichia coli:: Influence of growth medium osmolarity
复制标题
DOI：
10.1128/jb.181.10.3018-3024.1999
复制
发表时间：
1999-05-01
影响因子：
3.2
通讯作者：
Muñoa, FJ
中科院分区：
文献类型：
--
作者：
Balsalobre, C;Johansson, J;Muñoa, FJ
HHA蛋白属于一个新的调控家族，参与了毒力因子的环境调节。本工作的目的是用双向凝胶电泳法研究HHA突变对大肠杆菌细胞整体蛋白质图谱的影响。生长介质渗透压对HHA诱变效果有明显影响。与野生型细胞相比，HHA细胞在高渗透压下表达改变的蛋白质数是低渗透压下的3倍。在HHA等位基因改变表达的蛋白质中，可以鉴定到OmpA和磷酸转移酶系统的蛋白IIA(GLC)。由于后一种酶参与环状AMP合成的调控，从而影响分解代谢抑制系统，我们测试了LacZ基因在HHA突变体中的表达是否也发生了改变，这是很容易的，这表明至少HHA突变的部分多效性可能是由其对分解代谢抑制系统的影响引起的。
The Hha protein belongs to a new family of regulators involved in the environmental regulation of virulence factors. The aim of this work was to study the effect of the hha mutation on the overall protein pattern of Escherichia coli cells by two-dimensional polyacrylamide gel electrophoresis. The growth medium osmolarity clearly influenced the effect of the hha mutation. The number of proteins whose expression was altered in hha cells, compared with wild-type cells, was three times larger at a high osmolarity than at a low osmolarity, Among the proteins whose expression was modified by the hha allele, both OmpA and protein IIA(Glc) of the phosphotransferase system could be identified. As this latter enzyme participates in the regulation of the synthesis of cyclic AMP and hence influences the catabolite repression system, we tested whether the expression of the lacZ gene was also modified in hha mutants, This was the ease, suggesting that at least some of the pleiotropic effects of the hha mutation could be caused by its effect on the catabolite repression system.