The extracts of bredigite bioceramics enhanced the pluripotency of human dental pulp cells
The extracts of bredigite bioceramics enhanced the pluripotency of human dental pulp cells
复制标题
bredigite生物陶瓷提取物增强人牙髓细胞的多能性
DOI：
10.1002/jbm.a.36191
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发表时间：
2017
影响因子：
4.9
通讯作者：
Wei Xi
中科院分区：
文献类型：
--
作者：
Chen Lihong;Liu Lu;Wu Chengtie;Yang Ruiqi;Chang Jiang;Wei Xi
生物材料在组织工程和再生医学中有着深远的影响，但很少有研究报道生物陶瓷提取物在干细胞多能性调控中的作用。本研究研究了生物陶瓷提取物，包括硅酸盐bredigite （Ca7MgSi4O16）和传统的β‐三磷酸钙（β‐TCP），对人牙髓细胞（hDPCs）多能性和多系分化潜力的影响。碱性成纤维细胞生长因子（bFGF）是一种已知的hDPCs多能性调节因子，被用作参考。Bredigite提取物显著促进细胞生长、增殖、TERT表达，维持hDPCs处于存在状态。bredigite提取物显著上调Stro1、Oct4、Sox2等多能性相关基因的表达，进一步促进hDPCs在成牙/成脂诱导后的多系分化。bredigite提取物对hDPCs多能性的刺激与bFGF相当，而β‐TCP提取物缺乏这些特性。我们的研究结果首次表明，白牙石提取物增强了牙源性干细胞的多能性，为再生医学的广泛应用铺平了道路。©2017 Wiley期刊公司[J]中国生物医学工程学报，2016,35(5):559 - 564。
Biomaterials have a profound effect on tissue engineering and regenerative medicine, but few studies have reported the role of extracts from bioceramics in the regulation of stem cell pluripotency. The present study investigated the effects of bioceramics extracts, including silicate bredigite (Ca7MgSi4O16) and conventional β‐tricalcium phosphate (β‐TCP), on the pluripotency and the multilineage differentiation potential of human dental pulp cells (hDPCs). Basic fibroblast growth factor (bFGF), which is a known regulator of hDPCs pluripotency, was used as a reference. Bredigite extracts significantly promoted cell growth, proliferation, TERT expression and maintained hDPCs in a presenescent state. The extracts of bredigite significantly up‐regulated the expression of pluripotency‐related genes such as Stro1, Oct4 and Sox2, and further promoted the multilineage differentiation of hDPCs after odontogenic/adipogenic induction. The stimulation of bredigite extracts on hDPCs pluripotency was comparable to that of bFGF, whereas β‐TCP extracts lacked these properties. Our results suggested for the first time that bredigite extracts enhance the pluripotency of dental‐derived stem cells, paving the way for extended applications in regenerative medicine. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3465–3474, 2017.