A differential structure approach to membrane segmentation in electron tomography
A differential structure approach to membrane segmentation in electron tomography
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DOI：
10.1016/j.jsb.2011.05.010
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发表时间：
2011-09-01
影响因子：
3
通讯作者：
Fernandez, Jose-Jesus
中科院分区：
文献类型：
--
作者：
Martinez-Sanchez, Antonio;Garcia, Inmaculada;Fernandez, Jose-Jesus
电子断层扫描可以在分子细节上对细胞景观进行三维可视化。分割是解释重建的断层图像的重要阶段。虽然已经提出了几种计算方法，但没有一种作为通用方法流行，因此通过手动注释进行分割仍然是常见的选择。在这项工作中，我们介绍了一种分割方法，针对膜，它定义了生物标本内的车厢的自然限制。我们的方法是基于局部微分结构和高斯膜模型。首先，它通过尺度空间隔离信息，并在局部尺度上找到潜在的膜状点。然后，在全局范围内整合结构信息，以产生确定的分割。我们展示并验证了该算法的性能在不同的实验条件下的一些断层图像。(C)2011 Elsevier Inc. All rights reserved.
Electron tomography allows three-dimensional visualization of cellular landscapes in molecular detail. Segmentation is a paramount stage for the interpretation of the reconstructed tomograms. Although several computational approaches have been proposed, none has prevailed as a generic method and thus segmentation through manual annotation is still a common choice. In this work we introduce a segmentation method targeted at membranes, which define the natural limits of compartments within biological specimens. Our method is based on local differential structure and on a Gaussian-like membrane model. First, it isolates information through scale-space and finds potential membrane-like points at a local scale. Then, the structural information is integrated at a global scale to yield the definite segmentation. We show and validate the performance of the algorithm on a number of tomograms under different experimental conditions. (C) 2011 Elsevier Inc. All rights reserved.