STUDY OF BIOLOGICALLY RELEVANT PHYSICAL-CHEMICAL PROPERTIES OF BILE-SALTS BY REVERSE-PHASE LIQUID-CHROMATOGRAPHY
STUDY OF BIOLOGICALLY RELEVANT PHYSICAL-CHEMICAL PROPERTIES OF BILE-SALTS BY REVERSE-PHASE LIQUID-CHROMATOGRAPHY
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DOI：
10.1007/bf02688489
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发表时间：
1987-01-01
期刊：
影响因子：
1.7
通讯作者：
ALVARO, D
中科院分区：
文献类型：
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作者：
CANTAFORA, A;DIBIASE, A;ALVARO, D
胆汁酸盐在反相高效液相色谱柱上的色谱行为研究（C18和C8）和缓冲水-甲醇洗脱液系统表明，如果使用pH>7和0.154M盐水浓度的洗脱液，则辛醇-水分配系数和胶束胆固醇增溶能力与色谱迁移率线性相关（相关系数分别为0.98和0.86）。临界胶束浓度与各种测试条件下的色谱迁移率相关性很差。胆汁盐的pKa值由流动性随洗脱液pH的变化来确定。未结合和甘氨酸结合胆汁盐的值分别在4.92-5.06和4.27-4.47范围内，与先前的估计值一致。0.74-1.02范围内的值代表牛磺酸结合物的首次直接评价。
The study of chromatographic behaviour of bile salts with reverse-phase HPLC columns (C18 and C8) and buffered water — methanol eluent systems indicated that the octanol-water partition coefficient and the micellar cholesterol-solubilizing capacity are linearly correlated with the chromatographic mobilities if eluents with pH>7 and 0.154 M saline concentration are used (correlation coefficients 0.98 and 0.86 respectively). The critical micellar concentration was poorly correlated with the chromatographic mobility under the various conditions tested. The pKa values of bile salts were determined by the changes of mobility with the pH of eluent. Values in the range 4.92–5.06 and 4.27–4.47 were found respectively for unconjugated and glycineconjugated bile salt, in agreement with previous estimates. Values in the range 0.74–1.02 represent the first direct evaluation for taurine-conjugates.