Hierarchical Proteinosomes for Programmed Release of Multiple Components
Hierarchical Proteinosomes for Programmed Release of Multiple Components
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用于多种成分程序化释放的分层蛋白体
DOI：
10.1002/anie.201601427
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发表时间：
2016-06-13
影响因子：
16.6
通讯作者：
Mann, Stephen
中科院分区：
文献类型：
--
作者：
Liu, Xiaoman;Zhou, Pei;Mann, Stephen
描述了一种基于递归Pickering乳液程序使用两亲性蛋白质-聚合物纳米缀合物构建块的分层组织的多区室化蛋白质体的简易途径。在单个宿主蛋白质体内被嵌顿的客体蛋白质体的数量受到控制，并且酶和遗传聚合物被封装在靶向亚隔室中以产生化学组织的多层结构。三种类型的时空响应延迟伴随释放，同步释放或层次释放的葡聚糖和DNA的主机和客人的膜蛋白酶攻击的顺序响应的基础上证明，或通过变化的定位中的含二硫化物的交联在主机或客人的蛋白体集成到嵌套架构。总的来说，我们的研究提供了一个步骤，建设层次结构的合成原细胞与化学和空间整合的原细胞器。
A facile route to hierarchically organized multi-compartmentalized proteinosomes based on a recursive Pickering emulsion procedure using amphiphilic protein-polymer nanoconjugate building blocks is described. The number of incarcerated guest proteinosomes within a single host proteinosome is controlled, and enzymes and genetic polymers encapsulated within targeted subcompartments to produce chemically organized multi-tiered structures. Three types of spatiotemporal response-retarded concomitant release, synchronous release or hierarchical release of dextran and DNA-are demonstrated based on the sequential response of the host and guest membranes to attack by protease, or through variations in the positioning of disulfide-containing cross-links in either the host or guest proteinosomes integrated into the nested architectures. Overall, our studies provide a step towards the construction of hierarchically structured synthetic protocells with chemically and spatially integrated proto-organelles.