Olfactory Ionotropic Receptors in Mosquito Aedes albopictus (Diptera: Culicidae)
Olfactory Ionotropic Receptors in Mosquito Aedes albopictus (Diptera: Culicidae)
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DOI：
10.1093/jme/tjx063
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发表时间：
2017-09-01
影响因子：
2.1
通讯作者：
Wu, Wenjian
中科院分区：
文献类型：
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作者：
Chen, Qian;Man, Yahui;Wu, Wenjian
离子型谷氨酸受体 (iGluR) 是配体门控离子通道的保守家族，主要功能是介导突触处的神经元通讯。最近在昆虫中发现了 iGluR 的一个变异亚家族，即离子型受体 (IR)，并证明其具有气味识别功能。离子型受体参与独立于嗅觉受体活性的独特嗅觉信号传导途径。在本研究中，我们通过计算机比较序列分析，在白纹伊蚊 (Skuse) 中鉴定了 102 个假定的 IR 基因，称为 AalbIr 基因。 RT-PCR 表明，在 AalbIr 基因中，有 19 个基因在雌触角中表达。这些假定的嗅觉 AalbIR 共享四个保守的氨基酸疏水结构域，类似于传统 iGluR 中发现的跨膜和离子通道孔区域。为了确定这些嗅觉 AalbIR 在寻找宿主中的潜在功能，我们通过定量实时 RT-PCR 比较了它们在吸血雌性和非吸血雌性触角中的转录表达水平。当蚊子吃完血粉后，三个 AalbIr 基因表现出下调。这些结果可能有助于提高我们对蚊子中红外线介导的嗅觉信号的理解。
Ionotropic glutamate receptors (iGluRs) are a conserved family of ligand-gated ion channels that primarily function to mediate neuronal communication at synapses. A variant subfamily of iGluRs, the ionotropic receptors (IRs), was recently identified in insects and proved with the function in odorant recognition. Ionotropic receptors participate in a distinct olfactory signaling pathway that is independent of olfactory receptors activity. In the present study, we identify 102 putative IR genes, dubbed as AalbIr genes, in mosquito Aedes albopictus (Skuse) by in silico comparative sequence analysis. Among AalbIr genes, 19 show expression in the female antenna by RT-PCR. These putative olfactory AalbIRs share four conservative hydrophobic domains of amino acids, similar to the transmembrane and ion channel pore regions found in conventional iGluRs. To determine the potential function of these olfactory AalbIRs in host-seeking, we compared their transcript expression levels in the antennae of blood-fed females with that of non-blood-fed females by quantitative real-time RT-PCR. Three AalbIr genes showed downregulation when the mosquito finished a bloodmeal. These resultsmay help to improve our understanding of the IR-mediated olfactory signaling in mosquitoes.