Frequency-dependent inhibition of the intracellular calcium transients by calmodulin antagonists in the aequorin-injected rabbit papillary muscle.
Frequency-dependent inhibition of the intracellular calcium transients by calmodulin antagonists in the aequorin-injected rabbit papillary muscle.
复制标题
注射水母蛋白的兔乳头肌中钙调蛋白拮抗剂对细胞内钙瞬变的频率依赖性抑制。
DOI：
10.1007/978-1-4684-5679-0_49
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发表时间：
1989
影响因子：
--
通讯作者：
Endoh,M
中科院分区：
文献类型：
--
作者：
Endoh,M
钙调素是真核细胞中普遍存在的一种功能蛋白，在包括平滑肌细胞在内的多种组织中对酶和细胞过程的调节起重要作用（Cheung，1980; Klee和Newton，1985），虽然已经表明钙调蛋白存在于心肌细胞中并提示其可能参与心肌功能的调节，基于钙调素对酶活性和Ca 2+摄取的影响，其在完整心肌中的调节作用尚不清楚。为了解钙调素在心肌收缩中的作用，在离体兔乳头肌上，同时观察了钙调素拮抗剂W-7和三氟拉嗪（TFP）对细胞内钙瞬变和等长收缩的影响。
Calmodulin, a ubiquitous functional protein in eukaryotic cells, has been found to play an essential role in regulation of enzymes and cellular processes in various tissues including smooth muscle cells (Cheung, 1980; Klee and Newton, 1985), Although it has been shown that calmodulin exists in myocardial cells and suggested that it may be involved in regulation of myocardial functions, based on the effects of calmodulin on enzyme activities and Ca2+uptake, its regulatory role in intact myocardium remains unclear. In order to get insight into the role of calmodulin in regulation of myocardial contractility, the effects of calmodulin antagonists, W-7 and trifluoperazine (TFP), on the intracellular calcium transients and isometric contractions were assessed simultaneously in the isolated rabbit papillary muscle, superficial cells of which had been microinjected with the Ca2+sensitive bioluminescent protein aequorin.