Light and electron microscopic studies of intestinal epithelium in Notoplana humilis (Plathlminthes, Polycladida): The contribution of mesodermal/gastrodermal neoblasts to intestinal regeneration.
Light and electron microscopic studies of intestinal epithelium in Notoplana humilis (Plathlminthes, Polycladida): The contribution of mesodermal/gastrodermal neoblasts to intestinal regeneration.
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Notoplana humilis（Plathlminthes，Polycladida）肠上皮的光和电子显微镜研究：中胚层/胃真皮新生细胞对肠道再生的贡献。
DOI：
10.1007/s00441-015-2221-9
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
K. Kobayashi
中科院分区：
文献类型：
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作者：
D. Okano;S. Ishida;S. Ishiguro;K. Kobayashi
扁形蠕虫门中的一些自由生活的扁虫具有很强的再生能力，这种能力依赖于被认为是多能干细胞的新生母细胞。这些新生细胞的定义基于几个标准，包括它们的增殖能力和是否存在被称为类染色体的细胞成分。多壳类是一种海洋扁虫，有可能成为干细胞研究的良好模型系统，但关于新生细胞和再生的信息很少。在这项研究中，我们使用了抗组蛋白H3和BrdU的抗体，用透射电子显微镜和免疫组织化学染色的方法，鉴定了两类新母细胞：中胚层新生母细胞(位于间充质腔)和胃真皮新生母细胞(位于肠道内，颗粒俱乐部细胞和吞噬细胞也位于肠道内)。光镜和电子显微镜分析还表明，吞噬细胞和中胚层/胃胚层新生细胞，而不是颗粒俱乐部细胞，迁移到胚泡中，并在再生过程中重塑肠道。因此，我们认为，在多层结构中，肠道再生是通过变形(原有组织的重塑)和上形态(来自中胚层/胃真皮新生细胞的新生组织形成)两种机制来完成的。在假设来源于中胚层新生细胞的胃黏膜新生细胞是肠道干细胞的基础上，我们提出了一个研究肠道再生的模型。
Some free-living flatworms in the phylum Platyhelminthes possess strong regenerative capability that depends on putative pluripotent stem cells known as neoblasts. These neoblasts are defined based on several criteria, including their proliferative capacity and the presence of cellular components known as chromatoid bodies. Polyclads, which are marine flatworms, have the potential to be a good model system for stem cell research, yet little information is available regarding neoblasts and regeneration. In this study, transmission electron microscopy and immunostaining analyses, using antibodies against phospho-histone H3 and BrdU, were used to identify two populations of neoblasts in the polycladNotoplana humilis: mesodermal neoblasts (located in the mesenchymal space) and gastrodermal neoblasts (located within the intestine, where granular club cells and phagocytic cells are also located). Light and electron microscopic analyses also suggested that phagocytic cells and mesodermal/gastrodermal neoblasts, but not granular club cells, migrated into blastemas and remodeled the intestine during regeneration. Therefore, we suggest that, in polyclads, intestinal regeneration is accomplished by mechanisms underlying both morphallaxis (remodeling of pre-existing tissues) and epimorphosis (de novo tissue formation derived from mesodermal/gastrodermal neoblasts). Based on the assumption that gastrodermal neoblasts, which are derived from mesodermal neoblasts, are intestinal stem cells, we propose a model to study intestinal regeneration.