Aberrant expression during two‐stage mouse skin carcinogenesis of a type 147‐kDa keratin, k13, normally associated with terminal differentiation of internal stratified epithelia
Aberrant expression during two‐stage mouse skin carcinogenesis of a type 147‐kDa keratin, k13, normally associated with terminal differentiation of internal stratified epithelia
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147-kDa 型角蛋白 k13 在两阶段小鼠皮肤癌变过程中的异常表达，通常与内部复层上皮的终末分化相关
DOI：
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发表时间：
1988
影响因子：
4.6
通讯作者：
J. Schweizer
中科院分区：
文献类型：
--
作者：
R. Nischt;D. Roop;T. Mehrel;S. Yuspa;M. Rentrop;H. Winter;J. Schweizer
使用特异性角蛋白cDNA探针和单特异性抗角蛋白抗血清分析小鼠表皮和表皮肿瘤中I型47-kDa角蛋白K13的表达，K13通常与内部复层上皮的终末分化相关。我们证明，在发育的各个阶段，整个身体表皮几乎不存在这种角蛋白。此外，在各种形式的急性和慢性表皮过度增殖或在有利于细胞增殖或体外分化的条件下培养的表皮细胞中未检测到。相比之下，K13在由7，12-二甲基苯并[a]蒽和12-O-十四烷酰基佛波醇-13-乙酸酯（TPA）诱导的皮肤鳞状细胞癌中一致表达，而通过相同的两阶段方案获得的乳头状瘤在该角蛋白的表达方面明显异质。这些发现对两种不同品系的小鼠（NMRI和Sencar）都是正确的。从Sencar小鼠12周后收集的乳头状瘤或从NMRI小鼠15周后用TPA促进的乳头状瘤对K13呈阴性或引起不同量的这种角蛋白。在所有K13阳性表达的情况下，在肿瘤中，在正常的复层内上皮细胞，角蛋白及其mRNA总是发生在分化的细胞室。然而，与我们在内部复层上皮中发现的相反，K13的表达没有其常见的II型57 kDa伴侣K4。K13蛋白阴性的乳头状瘤也缺乏K13转录本。这表明肿瘤中K13的异常表达在转录水平上受到调节。我们的研究结果表明，K13可能提供了一个标志物恶性转化的小鼠两阶段皮肤癌模型，可能特别适合于基因表达调控的研究。
Specific keratin cDNA probes and monospecific antikeratin antisera were used to analyze mouse epidermis and epidermal tumors for the expression of a type I 47‐kDa keratin, K13, normally associated with terminal differentiation of internal stratified epithelia. We demonstrated that this keratin was virtually absent from the entire body epidermis at various stages of development. Also, it was not detected in various forms of acute and chronic epidermal hyperproliferation or in epidermal cells cultured under conditions that favored either cell proliferation or in vitro differentiation. In contrast, K13 was consistently expressed in squamous cell carcinomas of the skin induced by 7,12‐dimethylbenz[a]anthracene and 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), whereas papillomas obtained by the same two‐stage protocol were distinctly heterogeneous with regard to the expression of this keratin. These findings were true for two different strains of mice (NMRI and Sencar). Papillomas collected from Sencar mice after 12 wk or from NMRI mice after 15 wk of promotion with TPA were either negative for K13 or elicited variable amounts of this keratin. In all cases of positive expression of K13 in tumors, as in normal stratified internal epithelia, both the keratin protein and its mRNA invariably occurred in the differentiating cell compartments. In contrast to what we found in internal stratified epithelia, however, K13 was expressed without its commonly encountered type II 57‐kDa partner, K4. Papillomas negative for the K13 protein were also devoid of K13 transcripts. This indicates that the aberrant K13 expression in tumors is regulated at the level of transcription. Our results suggest that K13 may provide a marker for malignant conversion in the mouse two‐stage skin carcinogenesis model and may be especially suited for studies of gene expression regulation.
小鼠皮肤乳头状瘤中非整倍性、角蛋白修饰和γ-谷氨酰转移酶表达的连续发展。
影响因子：
11.2
作者：
Aldaz,CM;Conti,CJ;Larcher,F;Trono,D;Roop,DR;Chesner,J;Whitehead,T;Slaga,TJ
通讯作者：
Slaga,TJ
维生素 A 协调控制人角质形成细胞中角蛋白基因的表达。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gilfix,BM;Eckert,RL
通讯作者：
Eckert,RL
特定角蛋白作为具有分层上皮起源的肿瘤的分子标记。
影响因子：
11.2
作者：
Nelson,WG;Battifora,H;Santana,H;Sun,TT
通讯作者：
Sun,TT