Targeting and sensing cancer cells with ZnO nanoprobes in vitro
Targeting and sensing cancer cells with ZnO nanoprobes in vitro
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DOI：
10.1007/s10529-011-0641-5
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Lakshmi, Baddireddi Subhadra
中科院分区：
文献类型：
--
作者：
Sudhagar, Selvaraj;Sathya, Shanmugaraj;Lakshmi, Baddireddi Subhadra
研究了一类新型的氧化锌量子点（ZnO QDs）作为体外靶向癌细胞的纳米探针。采用溶胶-凝胶法制备了ZnO纳米粒子，并采用三甲氧基氨丙基硅烷对纳米粒子进行了包覆。将靶向癌细胞的配体转铁蛋白与ZnO量子点缀合。使用MDA-MB-231的体外成像研究表明，生物相容性ZnO纳米探针选择性地结合细胞表面受体并通过受体介导的内吞作用内化。时间推移的光漂白研究表明，ZnO量子点是耐光漂白，使它们适合于长期成像的目的。ZnO纳米探针作为敏感生物测定平台的研究表明，它可以用作癌细胞靶向和传感应用的替代荧光探针。
A new class of zinc oxide quantum dots (ZnO QDs) was investigated as nanoprobes for targeting cancer cells in vitro. ZnO nanoparticles were synthesized using conventional sol-gel method and encapsulated using trimethoxy aminopropyl silane. Transferrin, the ligand targeting the cancer cells, was conjugated to the ZnO QDs. In vitro imaging studies using MDA-MB-231 showed the biocompatible ZnO nanoprobe selectively binding to the cell surface receptor and internalizing through receptor-mediated endocytosis. Time-lapsed photobleaching studies indicate the ZnO QDs to be resistant to photobleaching, making them suitable for long term imaging purpose. Investigation of the ZnO nanoprobe as a platform for sensitive bioassays indicates that it can be used as an alternative fluoroprobe for cancer cell targeting and sensing applications.