Equine bone marrow-derived mesenchymal stromal cells (BMDMSCs) from the ilium and sternum: Are there differences?
Equine bone marrow-derived mesenchymal stromal cells (BMDMSCs) from the ilium and sternum: Are there differences?
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DOI：
10.1111/j.2042-3306.2012.00646.x
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发表时间：
2013-05-01
影响因子：
2.2
通讯作者：
McIlwraith, C. W.
中科院分区：
文献类型：
--
作者：
Adams, M. K.;Goodrich, L. R.;McIlwraith, C. W.
进行研究的原因在马中用于分离间充质基质细胞（MSC）的2个骨髓收获部位是胸骨和髂骨。技术程序是基于医生的偏好，但没有研究比较MSC浓度和生长速度之间的网站在25岁的马。目的本研究的目的是比较胸骨和髂骨之间以及连续5 ml骨髓穿刺液之间的有核细胞计数和生长率。我们假设胸骨中的MSC浓度高于髂骨，并且从任一部位的第一次连续抽吸将产生最大浓度的MSC。我们假设来自每个部位的细胞的生长速率不会有差异。方法7匹2 ~ 5岁马，连续2次从胸骨和髂骨取骨髓5 ml。在骨髓处理之前和之后获得有核细胞计数（NCC）。将细胞在培养物中扩增3代，并比较所有抽吸物的生长速率特征。结果两个部位第一次5 ml穿刺液的NCC均高于第二次5 ml穿刺液（P
Reasons for performing study The 2 sites of bone marrow harvest for isolation of mesenchymal stromal cells (MSC) in the horse are the sternum and ilium. The technical procedure is based on practitioner preference, but no studies have compared MSC concentrations and growth rates between the sites in horses aged 25 years. Objectives The objective of this study was to compare nucleated cell counts and growth rates between the sternum and ilium and between consecutive 5ml bone marrow aspirates. We hypothesised that there would be a higher concentration of MSCs in the sternum than the ilium, and that the first sequential aspirate from either site would yield the greatest concentration of MSCs. We hypothesised that growth rates of cells from each site would not differ. Methods Seven horses, aged 2 to 5 years, had 2 sequential 5ml marrow aspirates taken from the sternum and ilium. Nucleated cell counts (NCCs) were obtained before and after marrow processing. Cells were expanded in culture for 3 passages and growth rate characteristics compared for all aspirates. Results The NCCs of the first 5ml aspirate were higher than those of the second 5ml aspirate for both sites (P