FRIGIDA establishes a local chromosomal environment for FLOWERING LOCUS C mRNA production
FRIGIDA establishes a local chromosomal environment for FLOWERING LOCUS C mRNA production
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DOI：
10.1038/s41477-018-0250-6
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发表时间：
2018-10-01
期刊：
影响因子：
18
通讯作者：
He, Yuehui
中科院分区：
文献类型：
--
作者：
Li, Zicong;Jiang, Danhua;He, Yuehui
FRIGIDA（FRI）上调有效的花抑制因子FLOWERLOCUS C（FLC）的表达，赋予拟南芥冬季-周年生长习性：在长时间的冷暴露（春化）后加速向开花的过渡。在这里，我们表明，FRI，组蛋白乙酰转移酶，组蛋白甲基转移酶COMPASS样和其他染色质修饰剂的一部分，FRI含有超复合物富集在FLC转录起始位点（TSS）周围的区域，以促进其表达。几种FRI配偶体也富集在FLC侧翼的3'区域中，并且与FRI一起，它们起到增加TSS周围区域与3'区域物理缔合的频率并促进有义FLC和反义非编码RNA的表达的作用。我们的研究结果表明，FRI超复合物在FLC建立了一个局部的染色体环境，具有活性染色质修饰和拓扑结构，以促进转录激活，快速延伸和有效的前信使RNA剪接，导致高水平的FLC mRNA的产生。
FRIGIDA (FRI) upregulates the expression of the potent floral repressor FLOWERING LOCUS C (FLC) to confer the winter-annual growth habit in Arabidopsis thaliana: accelerated transition to flowering after prolonged cold exposure (vernalization). Here, we show that FRI, histone acetyltransferases, the histone methyltransferase COMPASS-like and other chromatin modifiers are part of a FRI-containing supercomplex enriched in a region around the FLC transcription start site (TSS) to promote its expression. Several FRI partners are also enriched in a 3' region flanking FLC and, together with FRI, they function to increase the frequency of physical association of the region around TSS with the 3' region and promote the expression of both sense FLC and antisense non-coding RNAs. Our results show that the FRI supercomplex establishes a local chromosomal environment at FLC with active chromatin modifications and topology to promote transcriptional activation, fast elongation and efficient premessenger RNA splicing, leading to a high-level production of FLC mRNAs.